Modulation of 8-methoxypsoralen-photoinduced cutaneous inflammatory reactions by various chemotherapeutic agents in vivo
| Autor: | Janak R. Kumar, Narendranath S. Ranadive, Herbert F. Haberman |
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| Rok vydání: | 1996 |
| Předmět: |
Indoles
Erythema Neutrophils medicine.medical_treatment Indomethacin Vascular permeability Inflammation Ibuprofen Pharmacology Biochemistry Capillary Permeability chemistry.chemical_compound In vivo medicine Animals Masoprocol Physical and Theoretical Chemistry PUVA Therapy Skin Single exposure Acetophenones General Medicine Nordihydroguaiaretic acid chemistry Quinacrine PUVA therapy Immunology Methoxsalen Rabbits medicine.symptom medicine.drug |
| Zdroj: | Photochemistry and photobiology. 63(4) |
| ISSN: | 0031-8655 |
| Popis: | Exposure of albino rabbits to UVA-VIS (320-700 nm) radiation after the topical application of 8-methoxypsoralen (8-MOP) cream is associated with acute cutaneous inflammatory reactions in situ. In the present studies the effects of various agents on 8-MOP plus light induced cutaneous inflammatory response viz. increase in vascular permeability (iVP), accumulation of polymorphonuclear leukocytes (aPMN) and erythema formation were investigated. The inflammatory reactions were induced by a single exposure of 8-MOP-sensitized sites to UVA-VIS (9.4J/cm2) light. Indomethacin, p-bromophenacyl bromide (BPAB), MK886 (trade name of Merck Sharpe & Dome), ibuprofen (IB), nordihydroguaiaretic acid (NDGA) or quinacrine were applied topically in cream base at various times prior to 8-MOP application. The iVP and aPMN were quantitated 24 h postirradiation using 125I-HSA and 51Cr-labeled PMN respectively, while erythema was graded visually. The rate of iVP, aPMN and erythema was inhibited almost completely by indomethacin (7.5-10%) when applied twice, 18 h and 3 h prior to 8-MOP. At lower concentrations of indomethacin (< or = 5%) iVP was inhibited whereas aPMN was augmented. The BPAB (0.25%) inhibited more than 90% of 8-MOP-photoinduced iVP and aPMN while there was partial reduction in erythema. The MK886 (0.1%) cream inhibited about 50% of iVP and aPMN but erythema persisted. The agents that are somewhat nonspecific such as IB, quinacrine and NDGA inhibited 8-MOP-photoinduced inflammation only marginally at the concentrations tested. The fact that iVP, aPMN and erythema can be dissociated suggests that there are independent variables in 8-MOP-photoinduced reactions, which involve multifactorial mechanisms probably controlled by different cell-signalling pathways and mediators. |
| Databáze: | OpenAIRE |
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