Integrated three-dimensional filter separates nanoscale from microscale elements in a microfluidic chip
| Autor: | Shane M. Eaton, Giulio Cerullo, Lorenzo Amato, Yu Gu, Roberto Osellame, Nicola Bellini |
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| Rok vydání: | 2012 |
| Předmět: |
Erythrocytes
Materials science Biomedical Engineering Bioengineering Nanotechnology Cell Separation 02 engineering and technology 01 natural sciences Biochemistry Intersection (Euclidean geometry) Rhodamine 6G chemistry.chemical_compound Humans Suspension (vehicle) Nanoscopic scale Microscale chemistry Rhodamines business.industry 010401 analytical chemistry Equipment Design General Chemistry Microfluidic Analytical Techniques 021001 nanoscience & nanotechnology Chip Microspheres 0104 chemical sciences chemistry Filter (video) Optoelectronics SPHERES 0210 nano-technology business Filtration |
| Zdroj: | Lab on a Chip Lab on a chip 12 (2012): 1135–1142. info:cnr-pdr/source/autori:L. Amato, Y. Gu, N. Bellini, S. M. Eaton, G. Cerullo, R. Osellame/titolo:Integrated three-dimensional filter separates nanoscale from microscale elements in a microfluidic chip/doi:/rivista:Lab on a chip (Print)/anno:2012/pagina_da:1135/pagina_a:1142/intervallo_pagine:1135–1142/volume:12 |
| ISSN: | 1473-0189 1473-0197 |
| DOI: | 10.1039/c2lc21116e |
| Popis: | We report on the integration of a size-based three-dimensional filter, with micrometre-sized pores, in a commercial microfluidic chip. The filter is fabricated inside an already sealed microfluidic channel using the unique capabilities of two-photon polymerization. This direct-write technique enables integration of the filter by post-processing in a chip that has been fabricated by standard technologies. The filter is located at the intersection of two channels in order to control the amount of flow passing through the filter. Tests with a suspension of 3 μm polystyrene spheres in a Rhodamine 6G solution show that 100% of the spheres are stopped, while the fluorescent molecules are transmitted through the filter. We demonstrate operation up to a period of 25 minutes without any evidence of clogging. Preliminary validation of the device for plasma separation from whole blood is shown. Moreover, the filter can be cleaned and reused by reversing the flow. |
| Databáze: | OpenAIRE |
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