Immobilization of laccase from Trametes versicolor on Lifetechtm supports for applications in degradation of industrial dyes
| Autor: | M Jelena Bebic, Marija Ćorović, Danica Mitrović, D Ana Milivojevic, I Dejan Bezbradica, M Katarina Banjanac, B Milica Simovic, Z Ana Vukoicic |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Immobilized enzyme
General Chemical Engineering 02 engineering and technology 010501 environmental sciences lcsh:Chemical technology 01 natural sciences Hydrophobic effect chemistry.chemical_compound Adsorption bioremediation Bromothymol blue lcsh:TP1-1185 0105 earth and related environmental sciences Trametes versicolor enzyme immobilization Laccase Bromocresol green biology Chemistry General Chemistry 021001 nanoscience & nanotechnology biology.organism_classification decolorization wastewater treatment Covalent bond textile dyes 0210 nano-technology Nuclear chemistry |
| Zdroj: | Hemijska Industrija, Vol 74, Iss 3, Pp 197-209 (2020) Hemijska industrija |
| ISSN: | 2217-7426 |
| Popis: | In this study, immobilization of laccase from Trametes versicolor on eight Lifetech™ supports, with different characteristics (pore size, length of the spacer arm and functional groups), was studied and optimized for intended use in bioremediation for decolorization of industrial wastewaters. Out of six tested amino-functionalized supports, the most promising carrier was proved to be porous Lifetech™ ECR8309F with primary amino groups and a C2 spacer arm. Onto this support, laccase is attached by forming electrostatic interactions so that the most active preparation has shown the activity of 66876 U/g support. On the other hand, during immobilization of laccase on epoxy-functionalized Lifetech™ ECR8285F, via hydrophobic interactions and covalent bonding confirmed by a desorption assay, immobilization yield of 60 % and the activity of 118929 U/g were accomplished. Furthermore, immobilized enzyme on this support showed high capacity for decolorization of dyes (Lanaset® Violet B, Lanaset® Blue 2R, bromothymol blue and bromocresol green), by combination of both adsorption and enzyme degradation. Decolorization was in the range of 88 to 96 % after 4 h, with more than 80 % achieved after only 45 min. Also, this preparation demonstrated high operational stability during seven consecutive reuses in all examined dye reaction systems.[Projects of the Serbian Ministry of Education, Science and Technological Development, Grant no. 451-03-68/2020-14/200135 and Grant no. 451-03-68/2020-14/ 200287] |
| Databáze: | OpenAIRE |
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