‘1-8 interferon inducible gene family’: putative colon carcinoma-associated antigens
| Autor: | Vered Daniel-Carmi, In-Sook Ahn, Matityahu Fridkin, M-S Do, G Lugassy, Ezra Vadai, Lior Carmon, Adrian Paz, Lea Eisenbach, Erez Bar-Haim, Arthur Machlenkin, Esther Tzehoval, Boaz Tirosh |
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| Rok vydání: | 2007 |
| Předmět: |
Cancer Research
Transgene medicine.medical_treatment Mice Transgenic Cross Reactions cytotoxic T lymphocyte Biology Mice Antigen Interferon Cell Line Tumor medicine Animals Humans Gene family Cytotoxic T cell Antigens Tumor-Associated Carbohydrate tumour immunity Gene Immunodominant Epitopes Beta-2 microglobulin Membrane Proteins Virology Molecular biology Cytokine colon cancer Oncology Colonic Neoplasms peptides Interferons Translational Therapeutics T-Lymphocytes Cytotoxic medicine.drug |
| Zdroj: | British Journal of Cancer |
| ISSN: | 1532-1827 0007-0920 |
| Popis: | D(b-/-)xbeta2 microglobulin (beta2m) null mice transgenic for a chimeric HLA-A2.1/D(b)-beta2m single chain (HHD mice) are an effective biological tool to evaluate the antitumour cytotoxic T-lymphocyte response of known major histocompatibility-restricted peptide tumour-associated antigens, and to screen for putative unknown novel peptides. We utilised HHD lymphocytes to identify immunodominant epitopes of colon carcinoma overexpressed genes. We screened with HHD-derived lymphocytes over 500 HLA-A2.1-restricted peptides derived from colon carcinoma overexpressed genes. This procedure culminated in the identification of seven immunogenic peptides, three of these were derived from the 'human 1-8D gene from interferon inducible gene' (1-8D). The 1-8D gene was shown to be overexpressed in fresh tumour samples. The three 1-8D peptides were both antigenic and immunogenic in the HHD mice. The peptides induce cytotoxic T lymphocytes that were able to kill a colon carcinoma cell line HCT/HHD, in vitro and retard its growth in vivo. One of the peptides shared by all the 1-8 gene family primed efficiently normal human cytotoxic T lymphocyte precursors. These results highlight the 1-8D gene and its homologues as putative immunodominant tumour-associated antigens of colon carcinoma. |
| Databáze: | OpenAIRE |
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