Oncogenic marek’s disease herpesvirus encodes an isoform of the conserved regulatory immediate early protein ICP27 generated by alternative promoter usage
| Autor: | Swantje Strassheim, Benoît Muylkens, Denis Rasschaert, Isabelle Gennart, S. Laurent, Marjolaine André |
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| Přispěvatelé: | UFR Sciences et Techniques, Université Francois Rabelais [Tours], Veterinary Integrated Research Unit, Faculty of Sciences, Namur Research Institute for Life Sciences (NARILIS), Université de Namur [Namur], Département Santé Animale (DEPT SA), Institut National de la Recherche Agronomique (INRA), Ligue contre le Cancer Grand Ouest – Comites départementaux 16, 35, 37 and 85 (France), Fonds Europeen de Developpement Regional (FEDER) EXMIR (no. 2835-34204) and Fonds de la recherche Scientifique(FNRS, grant FRFC J.0165.13, Methyllatency in Marek’s Disease Virus) (Belgium)., Université de Namur [Namur] (UNamur) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Gene Expression Regulation Viral ICP27 Isoform expression génique Transcription Genetic méthylation de l'adn viruses 030106 microbiology Biology Splicing marek ’ s disease virus Marek’s disease virus Immediate early protein Cell Line Gene product 03 medical and health sciences Viral Proteins Transcription (biology) Virology Gene expression Animals Protein Isoforms Promoter Regions Genetic Gene Regulation of gene expression promoteur promotor Epigenetic Promoter Herpesvirus 3. Good health Mardivirus 030104 developmental biology DNA methylation [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology gene expression virus maladie de marek Chickens |
| Zdroj: | Journal of General Virology Journal of General Virology, Microbiology Society, 2016, 97, pp.2399-2410. ⟨10.1099/jgv.0.000547⟩ |
| ISSN: | 0022-1317 1465-2099 |
| DOI: | 10.1099/jgv.0.000547 |
| Popis: | Herpesvirus gene expression is temporally regulated, with immediate early (IE), early (E) and late (L) genes. ICP27, which is involved in post-transcriptional regulation, is the only IE gene product conserved in all herpesviruses. We show here that the ICP27 transcript of the oncogenic Marek’s disease virus shares the same polyadenylation signal as the bicistronic glycoprotein K-ICP27 transcript and is regulated by alternative promoter usage, with transcription from its own promoter (pICP27) or that of gK (pgK). The pgK can generate a spliced ICP27 transcript yielding an N-terminal-deleted ICP27 isoform (ICP27DN) that, like ICP27, co-localizes with the SR protein in infected cells, but with a diffuse nuclear distribution. The pICP27 includes functional responsive elements (REs) for SP1, AP1 and CREB, is essentially active during the lytic phase and leads to exclusive expression of the native form of ICP27. The alternative promoter, pgK, including active REs for GATA, P53 and CREB, preferentially generates the gK transcript during the lytic phase and the spliced ICP27 transcript (ICP27DN) during the latent phase. An analysis of the DNA methylation marks of each promoter showed that pgK was systematically demethylated, whereas pICP27 was methylated during latency and demethylated during the lytic stage. Thus, MDV ICP27 gene expression is dependent on alternative promoters, the usage of which is regulated by DNA methylation, which differs between viral stages. |
| Databáze: | OpenAIRE |
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