Region 2 of the Escherichia coli K5 capsule gene cluster encoding proteins for the biosynthesis of the K5 polysaccharide
| Autor: | I S Roberts, Carlo Pazzani, Chantal Petit, Mark P. Stevens, Graham J. Boulnois, Annabel Smith, Veit Sieberth, Klaus Jann, Gordon P. Rigg |
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| Rok vydání: | 1995 |
| Předmět: |
Sequence analysis
Molecular Sequence Data Biology Uridine Diphosphate Glucose Dehydrogenase medicine.disease_cause Microbiology Bacterial Proteins Sequence Homology Nucleic Acid Gene cluster Escherichia coli medicine Transferase Amino Acid Sequence RNA Messenger Promoter Regions Genetic Molecular Biology Gene Peptide sequence Bacterial Capsules chemistry.chemical_classification Base Composition Base Sequence Sequence Homology Amino Acid Escherichia coli Proteins Polysaccharides Bacterial Nucleic acid sequence Chromosome Mapping Glycosyltransferases Sequence Analysis DNA Blotting Northern Molecular biology Amino acid chemistry Biochemistry Genes Bacterial Multigene Family |
| Zdroj: | Molecular Microbiology. 17:611-620 |
| ISSN: | 1365-2958 0950-382X |
| DOI: | 10.1111/j.1365-2958.1995.mmi_17040611.x |
| Popis: | The nucleotide sequence of region 2 of the Escherichia coli K5 capsule gene cluster has been determined. This region, essential for the biosynthesis of the K5 polysaccharide, contained four genes, termed kfiA-D. The G + C ratio was 33.4%, which was lower than the typical G + C ratio for E. coli and that of the flanking regions 1 and 3 in the K5 capsule gene cluster. Three major RNA transcripts were detected within region 2 by Northern blotting and three promoters located by transcript mapping. Promoter activity was confirmed by promoter-probe analysis. The predicted amino acid sequence of KfiC had homology to a number of glycosyl transferase enzymes and overexpression of the KfiC gene resulted in increased K5 transferase activity. The predicted amino acid sequence of KfiD had homology to a number of NAD-dependent dehydrogenase enzymes and was demonstrated to be a UDP-glucose dehydrogenase that catalyses the information of UDP-glucuronic acid from UDP-glucose. |
| Databáze: | OpenAIRE |
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