Water Dynamics in Cytoplasm-Like Crowded Environment Correlates with the Conformational Transition of the Macromolecular Crowder
| Autor: | Achintya Kundu, Pramod Kumar Verma, Minhaeng Cho, Jeong Hyon Ha |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
chemistry.chemical_classification
Nanotechnology 02 engineering and technology General Chemistry Polyethylene glycol Polymer 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Biochemistry Catalysis 0104 chemical sciences chemistry.chemical_compound Colloid and Surface Chemistry Water dynamics Solvation shell chemistry Cytoplasm Chemical physics Molecule 0210 nano-technology Macromolecular crowding Macromolecule |
| Zdroj: | Journal of the American Chemical Society. 138:16081-16088 |
| ISSN: | 1520-5126 0002-7863 |
| DOI: | 10.1021/jacs.6b10164 |
| Popis: | Polyethylene glycol (PEG) is a unique polymer material with enormous applicability in many industrial and scientific fields. Here, its use as macromolecular crowder to mimic the cellular environment in vitro is the focus of the present study. We show that femtosecond mid-IR pump–probe spectroscopy using three different IR probes, HDO, HN3, and azido-derivatized crowder, provides complete and stereoscopic information on water structure and dynamics in the cytoplasm-like macromolecular crowding environment. Our experimental results suggest two distinct subpopulations of water molecules: those that interact with other water molecules and those that are part of a hydration shell of crowder on its surface. Interestingly, water dynamics even in highly crowded environment remains bulk-like in spite of significant perturbation to the tetrahedral H-bonding network of water molecules. That is possible because of the formation of water aggregates (pools) even in water-deficient PEGDME-water solutions. In such a crow... |
| Databáze: | OpenAIRE |
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