In Vivo Targets of Pasteurella Multocida Toxin
| Autor: | Alistair J. Lax, Agamemnon E. Grigoriadis, Arshiya Banu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Pasteurella multocida toxin
animal diseases Proliferation Stimulation pHH3 medicine.disease_cause lcsh:Chemistry Pathogenesis Endometrium Mice Pasteurella multocida lcsh:QH301-705.5 Spectroscopy beta Catenin biology General Medicine respiratory system Immunohistochemistry Recombinant Proteins Computer Science Applications QE antibody G-proteins Female Antibody proliferation Ki67 β-catenin Signal Transduction G protein Bacterial Toxins Thymus Gland Catalysis Article Microbiology Inorganic Chemistry Bacterial Proteins In vivo otorhinolaryngologic diseases medicine Animals PHH3 Physical and Theoretical Chemistry Molecular Biology Cell Proliferation Toxin Organic Chemistry Uterus biology.organism_classification lcsh:Biology (General) lcsh:QD1-999 biology.protein GTP-Binding Protein alpha Subunits Gq-G11 Spleen |
| Zdroj: | International Journal of Molecular Sciences Banu, A, Lax, A J & Grigoriadis, A E 2020, ' In Vivo Targets of Pasteurella Multocida Toxin ', International Journal of Molecular Sciences, vol. 21, no. 8, 2739, pp. 2739-2750 . https://doi.org/10.3390/ijms21082739 International Journal of Molecular Sciences, Vol 21, Iss 2739, p 2739 (2020) Volume 21 Issue 8 |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms21082739 |
| Popis: | Many Pasteurella multocida strains are carried as commensals, while some cause disease in animals and humans. Some type D strains cause atrophic rhinitis in pigs, where the causative agent is known to be the Pasteurella multocida toxin (PMT). PMT activates three families of G-proteins&mdash Gq/11, G12/13, and Gi/o&mdash leading to cellular mitogenesis and other sequelae. The effects of PMT on whole animals in vivo have been investigated previously, but only at the level of organ-specific pathogenesis. We report here the first study to screen all the organs targeted by the toxin by using the QE antibody that recognizes only PMT-modified G-proteins. Under our experimental conditions, short-term treatment of PMT is shown to have multiple in vivo targets, demonstrating G-alpha protein modification, stimulation of proliferation markers and expression of active &beta catenin in a tissue- and cell-specific manner. This highlights the usefulness of PMT as an important tool for dissecting the specific roles of different G-alpha proteins in vivo. |
| Databáze: | OpenAIRE |
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