Small RNA genes expressed from Staphylococcus aureus genomic and pathogenicity islands with specific expression among pathogenic strains
| Autor: | Christophe Pichon, Brice Felden |
|---|---|
| Přispěvatelé: | Biochimie Pharmaceutique, Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Unité Propre de Recherche de l'Enseignement Supérieur JE 2311, Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Unité Propre de Recherche de l'Enseignement Supérieur JE 2311, Lefevre, Annick |
| Rok vydání: | 2005 |
| Předmět: |
Small RNA
Oligonucleotides MESH: Base Sequence MESH: Virulence Genome prokaryotes MESH: Oligonucleotides MESH: Sequence Analysis RNA MESH: Staphylococcus aureus MESH: Genomic Islands MESH: RNA Antisense Base Pairing Oligonucleotide Array Sequence Analysis Genetics MESH: Gene Expression Regulation Bacterial 0303 health sciences Multidisciplinary Virulence MESH: Genomics Genomics Biological Sciences 3. Good health Antisense RNA RNA Bacterial DNA Intergenic DNA microarray MESH: RNA Bacterial Staphylococcus aureus Genomic Islands In silico MESH: Base Pairing Molecular Sequence Data Biology 03 medical and health sciences small regulatory noncoding RNAs [SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular Biology nosocomial infections MESH: Blotting Northern [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology RNA Antisense Gene 030304 developmental biology MESH: DNA Intergenic MESH: Molecular Sequence Data Base Sequence Sequence Analysis RNA 030306 microbiology Gene Expression Regulation Bacterial Blotting Northern Pathogenicity island MESH: Oligonucleotide Array Sequence Analysis |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2005, 102 (40), pp.14249-54. ⟨10.1073/pnas.0503838102⟩ Proceedings of the National Academy of Sciences of the United States of America, 2005, 102 (40), pp.14249-54. ⟨10.1073/pnas.0503838102⟩ |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.0503838102 |
| Popis: | Small RNA (sRNA) genes are expressed in all organisms, primarily as regulators of translation and message stability. We have developed comparative genomic approaches to identify sRNAs that are expressed by Staphylococcus aureus , the most common cause of hospital-acquired infections. This study represents an in-depth analysis of the RNome of a Gram-positive bacterium. A set of sRNAs candidates were identified in silico within intergenic regions, and their expression levels were monitored by using microarrays and confirmed by Northern blot hybridizations. Two sRNAs were also detected directly from purification and RNA sequence determination. In total, at least 12 sRNAs are expressed from the S. aureus genome, five from the core genome and seven from pathogenicity islands that confer virulence and antibiotic resistance. Three sRNAs are present in multiple (two to five) copies. For the sRNAs that are conserved throughout the bacterial phylogeny, their secondary structures were inferred by phylogenetic comparative methods. In vitro binding assays indicate that one sRNA encoded within a pathogenicity island is a trans-encoded antisense RNA regulating the expression of target genes at the posttranscriptional level. Some of these RNAs show large variations of expression among pathogenic strains, suggesting that they are involved in the regulation of staphylococcal virulence. |
| Databáze: | OpenAIRE |
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