GENOTYPE ANALYSIS OF PREPRO-VASOPRESSIN SIGNAL PEPTIDE IN VASOPRESSIN-PRODUCING AND -NON-PRODUCING LUNG TUMORS
| Autor: | Masaru Shoji, Noriko Kimura, Hironobu Sasano, Takefumi Mori, Yutaka Kawarabayasi, Takayuki Sahata, Tohru Yamaji, Tokihisa Kimura, Miyuki Ishibashi, Takeharu Funyu, Kozo Ota, Masahiro Ohta |
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| Rok vydání: | 1997 |
| Předmět: |
Signal peptide
endocrine system Vasopressin Lung Neoplasms Genotype Vasopressins Molecular Sequence Data Mice Nude Protein Sorting Signals Biology Polymerase Chain Reaction General Biochemistry Genetics and Molecular Biology Mice Exon Tumor Cells Cultured Animals Humans Secretion Carcinoma Small Cell Protein Precursors General Pharmacology Toxicology and Pharmaceutics Base Sequence urogenital system Nucleic acid sequence DNA Restriction Enzymes Exons Sequence Analysis DNA General Medicine Molecular biology respiratory tract diseases Restriction enzyme nervous system Hypothalamus Cell culture Cancer research Neoplasm Transplantation hormones hormone substitutes and hormone antagonists |
| Zdroj: | Life Sciences. 61:2561-2566 |
| ISSN: | 0024-3205 |
| DOI: | 10.1016/s0024-3205(97)01010-2 |
| Popis: | A polymorphism in the nucleic acid sequence encoding the signal peptide of the human prepro-vasopressin (AVP) has been reported in an AVP producing small cell lung carcinoma (SCLC) cell line. The difference predicts expression in tumor cells of a variant signal peptide with Pro for Leu 11. To clarify whether this difference is required for AVP secretion from SCLC cells and/or reflects increased mutagenesis in malignant tumors, the exon encoding the signal peptide of prepro-AVP in two AVP producing SCLC and 9 non-producing lung tumors was amplified using polymerase chain reaction. The variant sequence was neither found by direct sequencing nor by restriction enzyme analysis. These results suggest that similar to the hypothalamus the normal signal peptide is functional in tumor cells and that the variant signal peptide is not a prerequisite for AVP secretion from SCLC cells. |
| Databáze: | OpenAIRE |
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