Amaranthus spinosusLinn. inhibits mast cell-mediated anaphylactic reactions
| Autor: | Sachin R Patel, Savita D. Patil, Sanjay J. Surana, Milap R Patel |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Male
medicine.medical_specialty Time Factors Cell Degranulation Immunology Acetates Toxicology Histamine Release Mice chemistry.chemical_compound Internal medicine Anti-Allergic Agents medicine Animals p-Methoxy-N-methylphenethylamine Mast Cells Rats Wistar Anaphylaxis Amaranthus Dose-Response Relationship Drug Plant Extracts Cell Membrane Degranulation Compound 48/80 medicine.disease Mast cell Rats Plant Leaves Disease Models Animal Endocrinology medicine.anatomical_structure chemistry Solvents Female Secretagogue Histamine Type I hypersensitivity |
| Zdroj: | Journal of Immunotoxicology. 9:77-84 |
| ISSN: | 1547-6901 1547-691X |
| DOI: | 10.3109/1547691x.2011.631609 |
| Popis: | The current study characterizes the mechanism by which the Amaranthus spinosus (Amaranthaceae) decreases mast cell-mediated anaphylactic reactions. Anaphylaxis is a typical hypersensitivity Type I reaction, sharing common mechanisms with asthma in its early and late phases. Mast cells are key as effector cells in hypersensitivity Type I reactions. A. spinosus has been traditionally used in the treatment of allergic bronchitis and asthma, but its role in mast cell-mediated anaphylactic reactions has not fully been investigated. This report investigated the potential effects of the ethyl acetate fraction of A. spinosus leaves (EAFAS) against a compound 48/80 (potent secretagogue)-induced systemic anaphylactic shock paradigm in a mouse model. In addition, rat peritoneal mast cells (RPMC) were used in in vitro studies to investigate the effect of EAFAS on compound 48/80-induced peritoneal mast cell degranulation and histamine release. When administration by the oral route-1 h before compound 48/80 injection-EAFAS (at dose from 0.001-1 g/kg) completely inhibited the induced anaphylactic shock. EAFAS at concentrations ranging 0.25-1 mg/ml dose-dependently attenuated rates of mast cell degranulation and histamine release from RPMC that were evoked by compound 48/80. The results of the present investigation indicated that EAFAS stabilizes the mast cell lipid bilayer membrane, thereby preventing the perturbation of membrane and the release of histamine. As a result of these anti-degranulating and anti-histaminic effects, it can be suggested that EAFAS may have a potential use in the prophylaxis and management of anaphylactic reactions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|