Expression Profile Analysis of the Low-Oxygen Response in Arabidopsis Root Cultures[W]
| Autor: | Erik Jan Klok, Iain W. Wilson, Dale L. Wilson, Scott Chapman, W. James Peacock, Shauna Somerville, Rudy Dolferus, Rob M. Ewing, Elizabeth S. Dennis |
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| Rok vydání: | 2002 |
| Předmět: |
Genetics
DNA Complementary Time Factors biology Gene Expression Profiling Arabidopsis Cell Biology Plant Science biology.organism_classification Plant Roots Oxygen Gene Expression Regulation Plant Regulatory sequence Culture Techniques Multigene Family Complementary DNA Transcriptional regulation DNA microarray Sequence motif Transcription factor Gene Research Article Oligonucleotide Array Sequence Analysis Signal Transduction |
| Zdroj: | The Plant Cell. 14:2481-2494 |
| ISSN: | 1532-298X |
| DOI: | 10.1105/tpc.004747 |
| Popis: | We used DNA microarray technology to identify genes involved in the low-oxygen response of Arabidopsis root cultures. A microarray containing 3500 cDNA clones was screened with cDNA samples taken at various times (0.5, 2, 4, and 20 h) after transfer to low-oxygen conditions. A package of statistical tools identified 210 differentially expressed genes over the four time points. Principal component analysis showed the 0.5-h response to contain a substantially different set of genes from those regulated differentially at the other three time points. The differentially expressed genes included the known anaerobic proteins as well as transcription factors, signal transduction components, and genes that encode enzymes of pathways not known previously to be involved in low-oxygen metabolism. We found that the regulatory regions of genes with a similar expression profile contained similar sequence motifs, suggesting the coordinated transcriptional control of groups of genes by common sets of regulatory factors. |
| Databáze: | OpenAIRE |
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