Detection of bacteraemias during non-surgicalroot canal treatment
| Autor: | W.P. Saunders, Jeremy Bagg, L. Savarrio, D. MacKenzie, Marcello P. Riggio |
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| Rok vydání: | 2004 |
| Předmět: |
Male
Pathology medicine.medical_specialty Sodium Hypochlorite Root canal Rubber Dams Bacteremia Biology Polymerase Chain Reaction Microbiology law.invention Root Canal Filling Materials law Root Canal Obturation medicine Pulsed-field gel electrophoresis Humans General Dentistry Polymerase chain reaction Pfge typing Electrophoresis Agar Gel Bacteriological Techniques Bacteria Root Canal Irrigants Dental procedures Chlorhexidine Potassium Iodide Hydrogen Peroxide Middle Aged medicine.disease Endodontics Electrophoresis Gel Pulsed-Field Root Canal Therapy medicine.anatomical_structure Infective endocarditis Anti-Infective Agents Local Female Detection rate Dental Pulp Cavity Gutta-Percha Dental Cavity Preparation |
| Zdroj: | Journal of dentistry. 33(4) |
| ISSN: | 0300-5712 |
| Popis: | Summary Some dental procedures initiate a bacteraemia. In certain compromised patients, this bacteraemia may lead to distant site infections, most notably infective endocarditis. Objective. To investigate whether a detectable bacteraemia was produced during non-surgical root canal therapy. Methods. Thirty patients receiving non-surgical root canal therapy were studied. Three blood samples were taken per patient: pre-operatively, peri-operatively and post-operatively. In addition, a paper point sample was collected from the root canal. The blood samples were cultured by pour plate and blood bottle methods. The isolated organisms were identified by standard techniques. Blood samples were analysed for the presence of bacterial DNA by the polymerase chain reaction (PCR). In two cases where the same species of organism was identified in the root canal and the bloodstream, the isolates were typed by pulsed field gel electrophoresis (PFGE). Results. By conventional culturing, a detectable bacteraemia was present in 9 (30%) of the 30 patients who had no positive pre-operative control blood sample. In 7 (23.3%) patients, the same species of organism was identified in both the bloodstream and in the paper point sample from the root canal system. Overall, PCR gave lower detection rates compared with conventional culture, with 10 of 90 (11%) of the blood samples displaying bacterial DNA. PFGE typing was undertaken for two pairs of culture isolates from blood and paper points; these were found to be genetically identical. Conclusions. Non-surgical root canal treatment may invoke a detectable bacteraemia. |
| Databáze: | OpenAIRE |
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