Two roles for Rad50 in telomere maintenance
| Autor: | Jean-Baptiste Vannier, Annie Depeiges, Maria Eugenia Gallego, Charles I. White |
|---|---|
| Přispěvatelé: | White, Charles, Génétique, Reproduction et Développement (GReD), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Telomerase
Indoles Mutant Arabidopsis [SDV.GEN] Life Sciences [q-bio]/Genetics medicine.disease_cause 0302 clinical medicine MESH: Arabidopsis MESH: Models Genetic MESH: Chromosomes Plant MESH: In Situ Hybridization Fluorescence Base Pairing In Situ Hybridization Fluorescence Anaphase MESH: Indoles 0303 health sciences Mutation General Neuroscience MESH: Telomerase Telomere MESH: Reproducibility of Results MESH: Cell Nucleus MESH: Mutation DNA Plant MESH: Base Pairing Flowers MESH: Arabidopsis Proteins Biology MESH: Anaphase General Biochemistry Genetics and Molecular Biology Article Chromosomes Plant 03 medical and health sciences medicine Sister chromatids MESH: DNA Plant Molecular Biology 030304 developmental biology Repetitive Sequences Nucleic Acid Cell Nucleus [SDV.GEN]Life Sciences [q-bio]/Genetics MESH: Repetitive Sequences Nucleic Acid General Immunology and Microbiology Models Genetic Arabidopsis Proteins Chromosome Reproducibility of Results MESH: Flowers Molecular biology Rad50 MESH: Telomere 030217 neurology & neurosurgery |
| Zdroj: | EMBO Journal EMBO Journal, 2006, 25 (19), pp.4577-85. ⟨10.1038/sj.emboj.7601345⟩ EMBO Journal, EMBO Press, 2006, 25 (19), pp.4577-85. ⟨10.1038/sj.emboj.7601345⟩ |
| ISSN: | 0261-4189 1460-2075 |
| DOI: | 10.1038/sj.emboj.7601345 |
| Popis: | International audience; We describe two roles for the Rad50 protein in telomere maintenance and the protection of chromosome ends. Using fluorescence in situ hybridisation (FISH) and fibre-FISH analyses, we show that absence of AtRad50 protein leads to rapid shortening of a subpopulation of chromosome ends and subsequently chromosome-end fusions lacking telomeric repeats. In the absence of telomerase, mutation of atrad50 has a synergistic effect on the number of chromosome end fusions. Surprisingly, this 'deprotection' of the shortened telomeres does not result in increased exonucleolytic degradation, but in a higher proportion of anaphase bridges containing telomeric repeats in atrad50/tert plants, compared to tert mutant plants. Absence of AtRad50 thus facilitates the action of recombination on these shortened telomeres. We propose that this protective role of Rad50 protein on shortened telomeres results from its action in constraining recombination to sister chromatids and thus avoiding end-to-end interactions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text