CIP2A immunosensor comprised of vertically-aligned carbon nanotube interdigitated electrodes towards point-of-care oral cancer screening
| Autor: | Shaowei Ding, Kshama Parate, Benjamin J. Brownlee, Taylor M. Davis, Loreen R. Stromberg, Suprem R. Das, Joseph Katz, Brian D. Iverson, Edward K. L. Chan, Jonathan C. Claussen |
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| Rok vydání: | 2018 |
| Předmět: |
Materials science
Point-of-Care Systems Biomedical Engineering Biophysics Nanotechnology 02 engineering and technology Carbon nanotube Chemical vapor deposition Biosensing Techniques 010402 general chemistry 01 natural sciences Autoantigens Antibodies law.invention law Limit of Detection Cancer Screening Tests Electrochemistry Humans Electrodes Early Detection of Cancer Point of care Detection limit Oral cancer screening Nanotubes Carbon Intracellular Signaling Peptides and Proteins Membrane Proteins General Medicine 021001 nanoscience & nanotechnology 0104 chemical sciences Interdigitated electrode Mouth Neoplasms 0210 nano-technology Biotechnology |
| Zdroj: | Biosensorsbioelectronics. 117 |
| ISSN: | 1873-4235 |
| Popis: | Vertically aligned carbon nanotube array (VANTA) coatings have recently garnered significant attention due in part to their unique material properties including light absorption, chemical inertness, and electrical conductivity. Herein we report the first use of VANTAs grown via chemical vapor deposition in a 2D interdigitated electrode (IDE) footprint with a high height-to-width aspect ratio (3:1 or 75:25 µm). The VANTA-IDEs were functionalized with an antibody (Ab) specific to the human cancerous inhibitor PP2A (CIP2A)—an oncoprotein that is associated with a variety of malignancies such as oral, breast, and multiple myeloma cancers. The resultant label-free immunosensor was capable of detecting CIP2A across a wide linear sensing range (1−100 pg/mL) with a detection limit of 0.24 pg/mL within saliva supernatant—a range that is more sensitive than the corresponding CIP2A enzyme linked immunosorbent assay (ELISA). These results help pave the way for rapid cancer screening tests at the point-of-care (POC) such as for the early-stage diagnosis of oral cancer at a dentist's office. |
| Databáze: | OpenAIRE |
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