Demonstration of Neutralizing Mucosal IgA Response to Intranasal HIV-1 env DNA Vaccines With or Without the V3 Glycosylation Site
| Autor: | Marlene Biller, Eric Rowcliffe, Sigvard Olofsson, Jorma Hinkula, Britta Wahren, Anders Bolmstedt |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Microbiology (medical)
Glycosylation Immunogen Enzyme-Linked Immunosorbent Assay V3 loop Biology Sensitivity and Specificity complex mixtures Statistics Nonparametric Virus DNA vaccination law.invention Mice Neutralization Tests Reference Values law Vaccines DNA Animals Primary isolate Administration Intranasal Probability AIDS Vaccines Immunity Cellular Mice Inbred BALB C General Immunology and Microbiology Immunogenicity virus diseases General Medicine Virology Immunoglobulin A Disease Models Animal Nasal Mucosa Infectious Diseases Immunology Recombinant DNA biology.protein Female Antibody |
| Zdroj: | Scandinavian Journal of Infectious Diseases. 36:360-364 |
| ISSN: | 1651-1980 0036-5548 |
| DOI: | 10.1080/00365540410020208 |
| Popis: | HIV-1 env based DNA vaccines are generally found to be poor B-cell immunogens. We examined the role of an N-glycan located in the V3 loop of HIV-1 (N306) that is known to modulate the immunogenicity of gp120. Here we describe intranasal immunizations with env (HIV-1 BRU) based genetic immunogens in combination with subcutaneous boosts of recombinant gp160 (rgp160) in mice. Immunization with DNA alone resulted in detectable IgA responses to rgp160 in both faeces and bronchoalveolar lavage (BAL) fluid, but the additional boosting increased the faecal IgA titres only. Protein boosting was required for induction of faecal IgA antibodies capable of neutralizing a homologous laboratory strain and a subtype B primary isolate. The B-cell response towards V3 loop peptides was not only directed against the homologous subtype B but also against the subtype F. In contrast to our previous observations on IgG, there were no differences in anti-gp160 IgA titres elicited by the N-glycan mutant and the wild-type immunogen. These results indicate that intranasal administration of plasmids containing env in combination with a subcutaneous boost proved to be an effective way of eliciting neutralizing mucosal IgA against HIV-1. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|