A Novel 3D in Vitro Tumor Model Based on Silk Fibroin/Chitosan Scaffolds To Mimic the Tumor Microenvironment
| Autor: | Fang Li, Chen-xi Qu, Shu-di Yang, Wei-liang Chen, Xue-nong Zhang, Yang Liu, Ben-gang You, Ji-zhao Li, Zhi-qiang Yuan, Ye-juan Zhou |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Materials science Lung Neoplasms Cell Drug Evaluation Preclinical Fibroin Antineoplastic Agents Biocompatible Materials 02 engineering and technology 03 medical and health sciences Drug Delivery Systems In vivo Cell Movement Cell Line Tumor medicine Tumor Microenvironment Humans General Materials Science Neoplasm Invasiveness A549 cell Tumor microenvironment Chitosan Microscopy Confocal Tissue Scaffolds Biomaterial 021001 nanoscience & nanotechnology In vitro 030104 developmental biology medicine.anatomical_structure Drug development A549 Cells Cancer research 0210 nano-technology Fibroins Porosity |
| Zdroj: | ACS applied materialsinterfaces. 10(43) |
| ISSN: | 1944-8252 |
| Popis: | Drug development involves various evaluation processes to ascertain drug effects and rigorous analysis of biological indicators during in vitro preclinical studies. Two-dimensional (2D) cell cultures are commonly used in numerous in vitro studies, which are poor facsimiles of the in vivo conditions. Recently, three-dimensional (3D) tumor models mimicking the tumor microenvironment and reducing the use of experimental animals have been developed generating great interest to appraise tumor response to treatment strategies in cancer therapy. In this study, silk fibroin (SF) protein and chitosan (CS), two natural biomaterials, were chosen to construct the scaffolds of 3D cell models. Human non-small cell lung cancer A549 cells in the SF/CS scaffolds were found to have a great tendency to gather and form tumor spheres. A549 cell spheres in the 3D scaffolds showed biological and morphological characteristics much closer to the in vivo tumors. Besides, the cells in 3D models displayed better invasion ability and drug resistance than 2D models. Additionally, differences in drug-resistant and immune-related protein levels were found, which indicated that 3D models might resemble the real-life situation. These findings suggested that these 3D tumor models composed of SF/CS are promising to provide a valuable biomaterial platform in the evaluation of anticancer drugs. |
| Databáze: | OpenAIRE |
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