Multigenerational exposure to silver ions and silver nanoparticles reveals heightened sensitivity and epigenetic memory in Caenorhabditis elegans
| Autor: | Carolin L. Schultz, Alison Crossley, Anye Wamucho, Jason M. Unrine, Claus Svendsen, Olga V. Tsyusko, David J. Spurgeon |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Silver Silver sulfide Metal Nanoparticles Nanotechnology 010501 environmental sciences 01 natural sciences Ecology and Environment General Biochemistry Genetics and Molecular Biology Silver nanoparticle Epigenesis Genetic 03 medical and health sciences chemistry.chemical_compound Animals Epigenetics Caenorhabditis elegans Metal nanoparticles Research Articles 0105 earth and related environmental sciences General Environmental Science Ions General Immunology and Microbiology biology General Medicine biology.organism_classification Engineered nanoparticles Biology and Microbiology 030104 developmental biology chemistry Nanotoxicology Biophysics General Agricultural and Biological Sciences |
| DOI: | 10.1098/rspb.2015.2911 |
| Popis: | The effects from multigenerational exposures to engineered nanoparticles (ENPs) in their pristine and transformed states are currently unknown despite such exposures being an increasingly common scenario in natural environments. Here, we examine how exposure over 10 generations affects the sensitivity of the nematode Caenorhabditis elegans to pristine and sulfidized Ag ENPs and AgNO 3 . We also include populations that were initially exposed over six generations but kept unexposed for subsequent four generations to allow recovery from exposure. Toxicity of the different silver forms decreased in the order AgNO 3 , Ag ENPs and Ag 2 S ENPs. Continuous exposure to Ag ENPs and AgNO 3 caused pronounced sensitization (approx. 10-fold) in the F2 generation, which was sustained until F10. This sensitization was less pronounced for Ag 2 S ENP exposures, indicating different toxicity mechanisms. Subtle changes in size and lifespan were also measured. In the recovery populations, the sensitivity to Ag ENPs and AgNO 3 resulting from the initial multigenerational exposure persisted. Their response sensitivity for all endpoints was most closely related to the last ancestral exposed generation (F5), rather than unexposed controls. The mechanisms of transgenerational transfer of sensitivity are probably organized through the epigenome, and we encourage others to investigate such effects as a priority for mechanistic toxicology. |
| Databáze: | OpenAIRE |
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