Thiolytic screening method for exploring condensed tannin variation in a unique sainfoin germplasm bank
| Autor: | J. Aufrere, C. Hayot, Katerina Theodoridou, Wayne Hayes, R.H. Brown, Elisabetta Stringano, Lydia M.J. Smith, Rainer Cramer, Irene Mueller-Harvey |
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| Přispěvatelé: | ProdInra, Migration, National Institute of Agricultural Botany (NIAB), Unité de recherche Plantes et Systèmes de Culture Horticoles (PSH), Institut National de la Recherche Agronomique (INRA), Unité de Recherches sur les Herbivores (URH), University of Reading (UOR), Tannin Community. Oxford, USA. |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Pharmaceutical Science
Fractionation 01 natural sciences Analytical Chemistry chemistry.chemical_compound Drug Discovery Botany Acetone [SDV.BV]Life Sciences [q-bio]/Vegetal Biology [SDV.BV] Life Sciences [q-bio]/Vegetal Biology Food science ComputingMilieux_MISCELLANEOUS Pharmacology chemistry.chemical_classification biology 010405 organic chemistry Chemistry Organic Chemistry biology.organism_classification 0104 chemical sciences 010404 medicinal & biomolecular chemistry Complementary and alternative medicine Onobrychis Thiolysis Molecular Medicine Composition (visual arts) Methanol Condensed tannin Prodelphinidin |
| Zdroj: | Queen's University Belfast-PURE Planta Medica 7. Tannin Conference 7. Tannin Conference, Tannin Community. Oxford, USA., Aug 2010, Berlin, Germany |
| Popis: | This EU funded 'HealthyHay'project stablished a sainfoin (Onobrychis vicifolia) germplasm bank at NIAB, Cambridge, with 306 accessions from around the world. A screening method was developed to characterise tannins by thiolytic degradation [1] directly in green plants for the first time. the method was validated by separate analysis of unextractable, extractable and purified tannins using thiolysis, HPLC-GPC and MALDI-TOF MS. Most tannins (58 to 73% of the total) could be recovered after Toyopearl HW50 fractionation with water, aqueous methanol and acetone. the greatest losses during purification occurred amongst larger molecular weight tannins with mean degree of polymerisation (mDP) > 18. The composition of water-,aqueous methanol- and acetone-soluble tannins differed considerably in their mDP and trans/cis ratios, but not in their prodelphinidin/orocyanidin (PD/PC) ratios. |
| Databáze: | OpenAIRE |
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