Putrescine regulates nitric oxide accumulation in Ganoderma lucidum partly by influencing cellular glutamine levels under heat stress
| Autor: | Liang Shi, Ang Ren, Jiale Xia, Mingwen Zhao, Jing Zhu, Shengli Wang, Chen-Gao Wu, Xiao-fei Han, Yanru Hu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Hot Temperature
Reishi Glutamine Biology Nitric Oxide Ornithine Decarboxylase Nitrate reductase Nitrate Reductase Microbiology Nitric oxide Ornithine decarboxylase 03 medical and health sciences chemistry.chemical_compound Biosynthesis Gene Expression Regulation Fungal Putrescine 030304 developmental biology 0303 health sciences 030306 microbiology Wild type Triterpenes chemistry Biochemistry Polyamine Heat-Shock Response |
| Zdroj: | Microbiological Research. 239:126521 |
| ISSN: | 0944-5013 |
| DOI: | 10.1016/j.micres.2020.126521 |
| Popis: | When fungi are subjected to abiotic stresses, the polyamines (PAs) level alter significantly. Here, we reveal that the polyamine putrescine (Put) could play an important role in alleviating heat stress(HS)-induced accumulation of nitric oxide (NO). Ornithine decarboxylase (ODC)-silenced mutants that were defective in Put biosynthesis exhibited significantly lower NO levels than the wild type (WT) when subjected to HS. With addition of 5 mM exogenous Put, the ODC-silenced mutant endogenous Put obviously increased under HS. At the same time, the contents of NO in the ODC-silenced mutants recovered to approximately WT levels after the administration of exogenous Put. However, the elevated NO content in the ODC-silenced mutants disappeared when exogenous Put and carboxy-PTIO (PTIO is a specific scavenger of NO) were added. Intriguingly, the content of glutamine (Gln) was significantly increased in the ODC-silenced strains. When exogenous Put was added to the WT, the Gln content was significantly decreased. The appearance of a high level of Gln was accompanied by nitrate reductase (NR) activity reduction. Further studies showed that Put influenced ganoderic acids (GAs) biosynthesis by regulating NO content, possibly through NR, under HS. Our work reported that Put regulates HS-induced NO accumulation by changing the cellular Gln level in filamentous fungi. IMPORTANCE: In our present work, it was HS as an ubiquitous environmental stress that affects the important pharmacological secondary metabolite (GAs) content in G. lucidum. Afterwards, we began to explore the network formed between multiple substances to jointly reduce the massive accumulation of GAs content caused by HS. We firstly focused on Put, a substance that enhances resistance to multiple stresses. Further, we discovered an influence on Put could changing the NO content, which has been shown to decrease the accumulation of GAs via HS. Then, we also found the change of NO content may be due to Put level that would affect intracellular Gln content. It has never been reported. And ultimately, it is Put related network that could reduce HS-inducing secondary metabolite mess in fungi. |
| Databáze: | OpenAIRE |
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