Identification of cancer stem-like side population cells in purified primary cultured human laryngeal squamous cell carcinoma epithelia

Autor: Chunping Wu, Shan Sun, Jie Tian, Jin-Yan Li, Huai-Dong Du, Liang Zhou, Ming Xie
Jazyk: angličtina
Rok vydání: 2013
Předmět:
Male
Cellular differentiation
lcsh:Medicine
Mice
SCID
Mice
Mice
Inbred NOD
Molecular Cell Biology
Basic Cancer Research
Tumor Cells
Cultured
Stem Cell Niche
lcsh:Science
Side-Population Cells
Multidisciplinary
medicine.diagnostic_test
Reverse Transcriptase Polymerase Chain Reaction
Stem Cells
Cell Differentiation
Flow Cytometry
Head and Neck Tumors
Adult Stem Cells
Oncology
Head and Neck Neoplasms
Carcinoma
Squamous Cell
Neoplastic Stem Cells
Medicine
Cellular Types
Research Article
Cell Survival
Blotting
Western
Biology
Flow cytometry
Head and Neck Squamous Cell Carcinoma
Microscopy
Electron
Transmission
Side population
Cancer stem cell
Cell Line
Tumor
medicine
Animals
Humans
Aged
Cell Proliferation
Squamous Cell Carcinoma of Head and Neck
Cell growth
lcsh:R
Cancers and Neoplasms
Cancer
medicine.disease
Molecular biology
Cell culture
Cancer cell
lcsh:Q
Developmental Biology
Zdroj: PLoS ONE, Vol 8, Iss 6, p e65750 (2013)
PLoS ONE
ISSN: 1932-6203
Popis: Cancer stem-like side population (SP) cells have been identified in many solid tumors; however, most of these investigations are performed using established cancer cell lines. Cancer cells in tumor tissue containing fibroblasts and many other types of cells are much more complex than any cancer cell line. Although SP cells were identified in the laryngeal squamous cell carcinoma (LSCC) cell line Hep-2 in our pilot study, it is unknown whether the LSCC tissue contains SP cells. In this study, LSCC cells (LSCCs) were primary cultured and purified from a surgically resected LSCC specimen derived from a well-differentiated epiglottic neoplasm of a Chinese male. This was followed by the verification of epithelium-specific characteristics, such as ultrastructure and biomarkers. A distinct SP subpopulation (4.45±1.07%) was isolated by Hoechst 33342 efflux analysis from cultured LSCCs by using a flow cytometer. Cancer stem cell (CSC)-associated assays, including expression of self-renewal and CSC marker genes, proliferation, differentiation, spheroid formation, chemotherapy resistance, and tumorigenicity were then conducted between SP and non-SP (NSP) LSCCs. In vitro and in vivo assays revealed that SP cells manifested preferential expression of self-renewal and CSC marker genes, higher capacity for proliferation, differentiation, and spheroid formation; enhanced resistance to chemotherapy; and greater xenograft tumorigenicity in immunodeficient mice compared with NSP cells. These findings suggest that the primary cultured and purified LSCCs contain cancer stem-like SP cells, which may serve as a valuable model for CSC research in LSCC.
Databáze: OpenAIRE
Externí odkaz: