Electrophysiological and autoradiographical evidence of V1 vasopressin receptors in the lateral septum of the rat brain
| Autor: | Eliane Tribollet, Jean-Jacques Dreifuss, Mario Raggenbass |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1987 |
| Předmět: |
Agonist
Male Vasopressin medicine.medical_specialty endocrine system Receptors Vasopressin medicine.drug_class Vasopressins Vasotocin Biology Oxytocin Tritium chemistry.chemical_compound Vasopressins/ physiology Internal medicine medicine Animals Neurons/drug effects/physiology Vasopressin receptor Arginine vasopressin receptor 1B Neurons Multidisciplinary Receptors Angiotensin Brain/drug effects/ physiology Brain Rats Inbred Strains Oxytocin receptor ddc:616.8 Rats Arginine Vasopressin Electrophysiology Oxytocin/metabolism Endocrinology chemistry nervous system Autoradiography Receptors Angiotensin/ physiology hormones hormone substitutes and hormone antagonists Antidiuretic medicine.drug Research Article Arginine Vasopressin/pharmacology |
| Zdroj: | Proceedings of the National Academy of Sciences, Vol. 84, No 21 (1987) pp. 7778-7782 |
| ISSN: | 0027-8424 |
| Popis: | Extracellular recordings were obtained from single neurons located in the lateral septum, an area known to receive a vasopressinergic innervation in the rat brain. Approximately half of the neurons tested responded to 8-L-arginine vasopressin (AVP) by a marked increase in firing rate at concentrations greater than 1 nM. The effect of vasopressin was blocked by synthetic structural analogues possessing antagonistic properties on peripheral vasopressin and oxytocin receptors. Oxytocin was much less potent than vasopressin in firing septal neurons, and a selective oxytocic agonist was totally ineffective. The action of vasopressin on neuronal firing was mimicked by the vasopressor agonist [2-phenylalanine,8-ornithine]vasotocin but not by the selective antidiuretic agonist 1-deamino[8-D-arginine]vasopressin. In a parallel study, sites that bind [3H]AVP at low concentration (1.5 nM) were found by in vitro autoradiography in the lateral septum. Adjacent sections were also incubated with 1.5 mM [3H]AVP and, in addition, with 100 nM [2-phenylalanine,8-ornithine]vasotocin or 1-deamino[8-D-arginine]vasopressin--i.e., the same compounds as those used for the electrophysiological study. Results showed that the vasopressor agonist, but not the antidiuretic agonist, displaced [3H]AVP, thus indicating that the vasopressin binding sites detected by autoradiography in the septum were V1 (vasopressor type) rather than V2 (antidiuretic type) receptors. Based on the electrophysiological evidence, we conclude that these receptors, when occupied, lead to increased firing of lateral septal neurons. |
| Databáze: | OpenAIRE |
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