Polymerase chain reaction using conjunctival swab samples for detecting Leishmania DNA in dogs
Autor: | Kamily Fagundes Pussi, Herintha Coeto Neitzke-Abreu, Lidiane Schultz Branquinho, Manoel Sebastião da Costa Lima Junior, Karen Araújo Magalhães, Elisabete Friozi, Silvia Barbosa do Carmo, Helton Krisman de Araújo |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Conjunctiva conjunctiva 030231 tropical medicine 030106 microbiology Biology Polymerase Chain Reaction SF1-1100 law.invention Lesion 03 medical and health sciences 0302 clinical medicine Dogs law medicine Canine leishmaniasis Parasite hosting Animals Dog Diseases Leishmania infantum Polymerase chain reaction Leishmania General Veterinary Leishmaniasis DNA DNA Protozoan medicine.disease biology.organism_classification DNA extraction eye diseases Animal culture medicine.anatomical_structure PCR Leishmaniasis Visceral Parasitology sense organs medicine.symptom |
Zdroj: | Revista Brasileira de Parasitologia Veterinária, Vol 30, Iss 3 (2021) |
ISSN: | 1984-2961 |
Popis: | The dog is the main domestic reservoir of Leishmania and font of infection for the vector, constituting an important host for the transmission of the parasite to humans. Non-invasive collection of swab samples for leishmaniasis diagnosis has been a promising alternative. This study analyzed the positivity of polymerase chain reaction (PCR) for the diagnosis of canine leishmaniasis in conjunctiva samples. DNA extraction was performed using SDS 20% and PCR was performed using 13A/13B primers that amplify 120-bp of Leishmania kDNA. Of the 77 dogs analyzed, 50 (64.93%) had ocular changes: 25 (32.47%) dogs had periocular lesion, 41 (53.25%) dogs had purulent eye discharge, and 17 (22.08%) dogs had both signals. PCR was positive in 35 dogs (45.45%), and there was no significant difference between dogs with and without ocular signals (p=0.4074). PCR positivity was significant higher in dogs without periocular injury (p=0.0018). Conjunctive PCR, a less invasive, fast, and painless collection technique, is indicated to complement the diagnosis, especially in dogs without periocular injury, independent of the presence of purulent eye discharge. |
Databáze: | OpenAIRE |
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