Utilisation of starch processing wastewater for production of microbial biomass protein and fungal α-amylase by Aspergillus oryzae
| Autor: | H. J. Van Leeuwen, Bo Jin, Qiming Yu, Bharat K. C. Patel |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
amylase
a-amylase enzyme Environmental Engineering protein synthesis Starch Aspergillus oryzae Bioengineering waste water management 060501 Bacteriology chemistry.chemical_compound Hydrolysis Air lift bioreactor Bioreactor Sarch processing wastewater Amylase Food science Waste Management and Disposal nonhuman biomass biology Renewable Energy Sustainability and the Environment business.industry Microbial biomass protein 060500 MICROBIOLOGY General Medicine Sterilization (microbiology) biology.organism_classification enzyme activity Biotechnology fungus growth 060000 BIOLOGICAL SCIENCES chemistry Wastewater enzyme synthesis biology.protein Single-cell protein business |
| Zdroj: | Bioresource Technology |
| ISSN: | 0960-8524 |
| DOI: | 10.1016/s0960-8524(98)00060-1 |
| Popis: | The production of microbial biomass protein (MBP) and fungal α-amylase from starch processing wastewater (SPW) by a selected strain Aspergillus oryzae was investigated as part of a comprehensive SPW treatment programme. A simple, non-aseptic, one-stage process was successfully carried out by using an air lift bioreactor under the optimised growth conditions at pH 5·0 and 35°C. Bioproduct yields of 6·1 g/litre of MBP that contained 38% protein, and 55 EU/ml of α-amylase enzyme were obtained from a 12-h batch culture. The pellet bioproduct led to easy harvesting of the biomass protein. The SPW was treated by removing 95% COD, 93% BOD and 98% suspended solids, and can be used for farm irrigation. The α-amylase had a stable enzyme activity at pH 5–9 and 25–35°C. Pretreatment by hydrolysis and sterilization of SPW, and nutrient supplementation may be unnecessary. |
| Databáze: | OpenAIRE |
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