A comparative analysis of drinking water employing metagenomics

Autor: Anwar Huq, Joseph A. Cotruvo, Rita R. Colwell, Kyle D. Brumfield, Shah M. Rashed, Menu B. Leddy, Nur A. Hasan
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Microorganism
Colony Count
Microbial
010501 environmental sciences
01 natural sciences
Natural Resources
Bacteriophages
Food science
Principal Component Analysis
Multidisciplinary
Virulence
biology
Microbiota
Genomics
Bottled water
6. Clean water
Spring
Chemistry
Medical Microbiology
Viruses
Physical Sciences
Water Resources
Medicine
Seasons
Chlorine
Proteobacteria
Research Article
Chemical Elements
Science
Indicator bacteria
Microbial Genomics
Microbiology
Actinobacteria
03 medical and health sciences
Surface Water
Genetics
Microbiome
0105 earth and related environmental sciences
Bacteria
Drinking Water
Ecology and Environmental Sciences
Organisms
Biology and Life Sciences
DNA
biology.organism_classification
030104 developmental biology
Genes
Bacterial
Metagenomics
Earth Sciences
Hydrology
Zdroj: PLoS ONE, Vol 15, Iss 4, p e0231210 (2020)
PLoS ONE
ISSN: 1932-6203
Popis: The microbiological content of drinking water traditionally is determined by employing culture-dependent methods that are unable to detect all microorganisms, especially those that are not culturable. High-throughput sequencing now makes it possible to determine the microbiome of drinking water. Thus, the natural microbiota of water and water distribution systems can now be determined more accurately and analyzed in significantly greater detail, providing comprehensive understanding of the microbial community of drinking water applicable to public health. In this study, shotgun metagenomic analysis was performed to determine the microbiological content of drinking water and to provide a preliminary assessment of tap, drinking fountain, sparkling natural mineral, and non-mineral bottled water. Predominant bacterial species detected were members of the phyla Actinobacteria and Proteobacteria, notably the genera Alishewanella, Salmonella, and Propionibacterium in non-carbonated non-mineral bottled water, Methyloversatilis and Methylibium in sparkling natural mineral water, and Mycobacterium and Afipia in tap and drinking fountain water. Fecal indicator bacteria, i.e., Escherichia coli or enterococci, were not detected in any samples examined in this study. Bacteriophages and DNA encoding a few virulence-associated factors were detected but determined to be present only at low abundance. Antibiotic resistance markers were detected only at abundance values below our threshold of confidence. DNA of opportunistic plant and animal pathogens was identified in some samples and these included bacteria (Mycobacterium spp.), protozoa (Acanthamoeba mauritaniensis and Acanthamoeba palestinensis), and fungi (Melampsora pinitorqua and Chryosporium queenslandicum). Archaeal DNA (Candidatus Nitrosoarchaeum) was detected only in sparkling natural mineral water. This preliminary study reports the complete microbiome (bacteria, viruses, fungi, and protists) of selected types of drinking water employing whole-genome high-throughput sequencing and bioinformatics. Investigation into activity and function of the organisms detected is in progress.
Databáze: OpenAIRE
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