Interobserver variability of cervical cytology in HIV-infected women
| Autor: | Valérie Potard, I. Cartier, Isabelle Heard, C. Bergeron, Dominique Costagliola |
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| Rok vydání: | 2014 |
| Předmět: |
Adult
medicine.medical_specialty Histology Human immunodeficiency virus (HIV) Papanicolaou stain Cervix Uteri medicine.disease_cause Sensitivity and Specificity Pathology and Forensic Medicine Cohort Studies Young Adult Cytology HIV Seropositivity Medicine Humans Multicenter Studies as Topic Gynecology Observer Variation Vaginal Smears Reproducibility business.industry Obstetrics Papillomavirus Infections Reproducibility of Results Cervical cytology General Medicine Uterine Cervical Dysplasia Confidence interval Liquid-based cytology Female business Kappa Papanicolaou Test |
| Zdroj: | Cytopathology : official journal of the British Society for Clinical Cytology. 26(6) |
| ISSN: | 1365-2303 |
| Popis: | Objectives Our objectives were to determine the reproducibility of cytological specimen interpretation between two pathologists in human immunodeficiency virus (HIV)-infected women (from the VIHGY, ANRS CO17 study of human papillomavirus genital pathology among HIV-positive women) and to analyse the improvement, if any, between conventional and liquid-based cytology (LBC) interpretations. Materials and methods A sample of all abnormal and 40% of randomly selected normal Papanicolaou (Pap) tests was randomly ordered and read blindly by a second pathologist using the revised Bethesda terminology 2001. For both conventional and liquid-based preparations, unweighted and Cicchetti–Allison-weighted kappa and their 95% confidence intervals (CIs) were calculated. Kappa values were then compared using the Altman rule to classify the reproducibility of cytological specimen interpretation. Results Two hundred and seventy-seven conventional Pap tests were reviewed, including 79 abnormal and 10 unsatisfactory results. Overall agreement between the two observers was 78%, with an estimated Cicchetti–Allison-weighted kappa of 0.69 (95%CI, 0.61–0.77). The corresponding values for the 268 LBCs, including 123 abnormal and two unsatisfactory results, were 84% and 0.82 (95%CI, 0.76–0.87), respectively. The reproducibility of LBC interpretations was significantly higher than that of conventional preparations (P = 0.009) and, for both laboratories, the percentages of unsatisfactory results were significantly lower for LBC. Conclusion In HIV-infected women in the combination antiretroviral therapy era, the strength of agreement was better for LBCs than for conventional preparations, with a lower percentage of unsatisfactory results. When available, LBC should be preferred because of its higher reproducibility. |
| Databáze: | OpenAIRE |
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