Effects of treadmill exercise on anxiety-like behavior in association with changes in estrogen receptors ERα, ERβ and oxytocin of C57BL/6J female mice
Autor: | Zhen Tian, Bin Yu, Xiao-Xia Cheng, Feng-Qin He, Zi-Jian Wang, Mei-Yang Fan, Rui-Juan Lai, Bing-Jie Yan, Yu-Nan Hui, Ming-Juan Yang, Xin Chen |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
medicine.medical_specialty
OF open field test TRE treadmill exercise E2 17-beta-oestradiol Estrogen receptor Treadmill exercise Neurosciences. Biological psychiatry. Neuropsychiatry Amygdala Supraoptic nucleus Open field HRP horseradishperoxidase HTRE higher speed TRE Chronic variable moderate stress (CVMS) mPOA medial preopticarea Estrogen receptor β (ERβ) Internal medicine Estrogen receptor α (ERα) Treadmill exercise (TRE) ERβ-IRs estrogen receptor β immunoreactive neurons Medicine BNST bed nucleus of the stria terminalis LMTRE low-moderate speed TRE business.industry CVMS chronic variable moderate stress General Neuroscience MeA medial amygdaloid nucleus ELISA enzyme-linked immunosorbent assay PBS phosphatebufferedsolution EPM elevated plusmazetest HPA hypothalamic–pituitary–adrenal SON supraoptic nucleus Stria terminalis medicine.anatomical_structure Endocrinology Oxytocin OT-IRs Oxytocin immunoreactive neurons ERα-IRs estrogen receptors αimmunoreactive neurons Oxytocin (OT) business Nucleus hormones hormone substitutes and hormone antagonists Research Paper PVN paraventricular nucleus medicine.drug RC321-571 |
Zdroj: | IBRO Neuroscience Reports, Vol 11, Iss, Pp 164-174 (2021) IBRO Neuroscience Reports |
ISSN: | 2667-2421 |
Popis: | Exercise can reduce the incidence of stress-related mental diseases, such as depression and anxiety. Control group was neither exposed to CVMS nor TRE (noCVMS/noTRE). Females were tested and levels of serum17-beta-oestradiol (E2), estrogen receptors α immunoreactive neurons (ERα-IRs), estrogen receptors β immunoreactive neurons (ERβ-IRs) and oxytocin immunoreactive neurons (OT-IRs) were measured. The results showed there’s increased anxiety-like behaviors for mice from CVMS/noTRE, CVMS/higher speed TRE (CVMS/HTRE) and noCVMS/HTRE groups when they were put in open field and elevated maze tests. They had lower serum E2 levels than mice from CVMS/low-moderate speed TRE (CVMS/LMTRE), noCVMS/LMTRE and noCVMS/noTRE groups. The three groups of CVMS/noTRE, CVMS/HTRE and noCVMS/HTRE mice had more ERα-IRs and less ERβ-IRs in the medial preoptic area (mPOA), bed nucleus of the stria terminalis (BNST) and medial amygdala (MeA), hypothalamic paraventricular nucleus (PVN) and supraoptic nucleus (SON). The number of OT-IRs in PVN and SON of CVMS/noTRE, CVMS/HTRE and noCVMS/HTRE mice was also lower than that of mice from CVMS/LMTRE, noCVMS/LMTRE and noCVMS/noTRE groups. Interestingly, CVMS/LMTRE and noCVMS/LMTRE mice were similar to noCVMS/noTRE mice in that they did not show anxiety, while CVMS/HTRE and noCVMS/HTRE mice did not, which were similar to the mice in CVMS/noTRE. We propose that LMTRE instead of HTRE changes the serum concentration of E2. ERβ/ERα ratio and OT level in the brain may be responsible for the decrease in anxiety-like behavior in female mice exposed to anxiety-inducing stress conditions. Highlights • CVMS/LMTRE did not show anxiety. • noCVMS/LMTRE did not show anxiety. • ERβ/ERα ratio decreas anxiety. • OT decreas anxiety. |
Databáze: | OpenAIRE |
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