Effects of treadmill exercise on anxiety-like behavior in association with changes in estrogen receptors ERα, ERβ and oxytocin of C57BL/6J female mice

Autor: Zhen Tian, Bin Yu, Xiao-Xia Cheng, Feng-Qin He, Zi-Jian Wang, Mei-Yang Fan, Rui-Juan Lai, Bing-Jie Yan, Yu-Nan Hui, Ming-Juan Yang, Xin Chen
Jazyk: angličtina
Rok vydání: 2021
Předmět:
medicine.medical_specialty
OF
open field test
TRE
treadmill exercise
E2
17-beta-oestradiol
Estrogen receptor
Treadmill exercise
Neurosciences. Biological psychiatry. Neuropsychiatry
Amygdala
Supraoptic nucleus
Open field
HRP
horseradishperoxidase
HTRE
higher speed TRE
Chronic variable moderate stress (CVMS)
mPOA
medial preopticarea
Estrogen receptor β (ERβ)
Internal medicine
Estrogen receptor α (ERα)
Treadmill exercise (TRE)
ERβ-IRs
estrogen receptor β immunoreactive neurons
Medicine
BNST
bed nucleus of the stria terminalis
LMTRE
low-moderate speed TRE
business.industry
CVMS
chronic variable moderate stress
General Neuroscience
MeA
medial amygdaloid nucleus
ELISA
enzyme-linked immunosorbent assay
PBS
phosphatebufferedsolution
EPM
elevated plusmazetest
HPA
hypothalamic–pituitary–adrenal
SON
supraoptic nucleus
Stria terminalis
medicine.anatomical_structure
Endocrinology
Oxytocin
OT-IRs
Oxytocin immunoreactive neurons
ERα-IRs
estrogen receptors αimmunoreactive neurons
Oxytocin (OT)
business
Nucleus
hormones
hormone substitutes
and hormone antagonists
Research Paper
PVN
paraventricular nucleus
medicine.drug
RC321-571
Zdroj: IBRO Neuroscience Reports, Vol 11, Iss, Pp 164-174 (2021)
IBRO Neuroscience Reports
ISSN: 2667-2421
Popis: Exercise can reduce the incidence of stress-related mental diseases, such as depression and anxiety. Control group was neither exposed to CVMS nor TRE (noCVMS/noTRE). Females were tested and levels of serum17-beta-oestradiol (E2), estrogen receptors α immunoreactive neurons (ERα-IRs), estrogen receptors β immunoreactive neurons (ERβ-IRs) and oxytocin immunoreactive neurons (OT-IRs) were measured. The results showed there’s increased anxiety-like behaviors for mice from CVMS/noTRE, CVMS/higher speed TRE (CVMS/HTRE) and noCVMS/HTRE groups when they were put in open field and elevated maze tests. They had lower serum E2 levels than mice from CVMS/low-moderate speed TRE (CVMS/LMTRE), noCVMS/LMTRE and noCVMS/noTRE groups. The three groups of CVMS/noTRE, CVMS/HTRE and noCVMS/HTRE mice had more ERα-IRs and less ERβ-IRs in the medial preoptic area (mPOA), bed nucleus of the stria terminalis (BNST) and medial amygdala (MeA), hypothalamic paraventricular nucleus (PVN) and supraoptic nucleus (SON). The number of OT-IRs in PVN and SON of CVMS/noTRE, CVMS/HTRE and noCVMS/HTRE mice was also lower than that of mice from CVMS/LMTRE, noCVMS/LMTRE and noCVMS/noTRE groups. Interestingly, CVMS/LMTRE and noCVMS/LMTRE mice were similar to noCVMS/noTRE mice in that they did not show anxiety, while CVMS/HTRE and noCVMS/HTRE mice did not, which were similar to the mice in CVMS/noTRE. We propose that LMTRE instead of HTRE changes the serum concentration of E2. ERβ/ERα ratio and OT level in the brain may be responsible for the decrease in anxiety-like behavior in female mice exposed to anxiety-inducing stress conditions.
Highlights • CVMS/LMTRE did not show anxiety. • noCVMS/LMTRE did not show anxiety. • ERβ/ERα ratio decreas anxiety. • OT decreas anxiety.
Databáze: OpenAIRE
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