Global Proteomic Profiling of Pediatric AML: A Pilot Study
| Autor: | Junmin Peng, Haiyan Tan, Xueyuan Cao, Jatinder K. Lamba, Huiyun Wu, Stanley Pounds, Nam H.K. Nguyen, Jeffrey E. Rubnitz |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Cancer Research Biology Proteomics Article Transcriptome 03 medical and health sciences pediatrics acute myeloid leukemia leukemic cells transcriptomics 0302 clinical medicine hemic and lymphatic diseases medicine CD90 tandem mass tag RC254-282 Proteomic Profiling untargeted labeled proteomics Myeloid leukemia Neoplasms. Tumors. Oncology. Including cancer and carcinogens Minimal residual disease 030104 developmental biology Oncology 030220 oncology & carcinogenesis Proteome Cancer research Cytarabine medicine.drug |
| Zdroj: | Cancers, Vol 13, Iss 3161, p 3161 (2021) Cancers Volume 13 Issue 13 |
| ISSN: | 2072-6694 |
| Popis: | Acute Myeloid Leukemia (AML) is a heterogeneous disease with several recurrent cytogenetic abnormalities. Despite genomics and transcriptomics profiling efforts to understand AML’s heterogeneity, studies focused on the proteomic profiles associated with pediatric AML cytogenetic features remain limited. Furthermore, the majority of biological functions within cells are operated by proteins (i.e., enzymes) and most drugs target the proteome rather than the genome or transcriptome, thus, highlighting the significance of studying proteomics. Here, we present our results from a pilot study investigating global proteomic profiles of leukemic cells obtained at diagnosis from 16 pediatric AML patients using a robust TMT-LC/LC-MS/MS platform. The proteome profiles were compared among patients with or without core binding factor (CBF) translocation indicated by a t(8 21) or inv(16) cytogenetic abnormality, minimal residual disease status at the end of the first cycle of chemotherapy (MRD1), and in vitro chemosensitivity of leukemic cells to cytarabine (Ara-C LC50). Our results established proteomic differences between CBF and non-CBF AML subtypes, providing insights to AML subtypes physiology, and identified potential druggable proteome targets such as THY1 (CD90), NEBL, CTSF, COL2A1, CAT, MGLL (MAGL), MACROH2A2, CLIP2 (isoform 1 and 2), ANPEP (CD13), MMP14, and AK5. |
| Databáze: | OpenAIRE |
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