Tobacco proteinase inhibitor I genes are locally, but not systemically induced by stress
| Autor: | J. F. Bol, C. van der Does, Frans Th. Brederode, Huub J. M. Linthorst |
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| Rok vydání: | 1993 |
| Předmět: |
Serine Proteinase Inhibitors
Nicotiana tabacum Molecular Sequence Data Plant Science Polymerase Chain Reaction chemistry.chemical_compound Complementary DNA Tobacco Gene expression Genetics Tobacco mosaic virus Amino Acid Sequence Cloning Molecular Plant Proteins Base Sequence Sequence Homology Amino Acid biology cDNA library fungi food and beverages Tobamovirus DNA General Medicine biology.organism_classification Molecular biology Kinetics Plants Toxic Gene Expression Regulation chemistry Agronomy and Crop Science Solanaceae Ethephon |
| Zdroj: | Plant Molecular Biology. 21:985-992 |
| ISSN: | 1573-5028 0167-4412 |
| DOI: | 10.1007/bf00023597 |
| Popis: | A cDNA library of tobacco mosaic virus (TMV)-infected tobacco was screened with polymerase chain reaction products obtained using a degenerate primer corresponding to proteinase inhibitor I (PI-I) of tomato and potato. The resulting clones encoded two highly similar, putative tobacco PI-I proteins, indicating that both genes identified in tobacco are probably expressed. The tobacco PI-I's were approximately 50% identical to wound-inducible potato and tomato PI-I and 80% identical to an ethylene-regulated tomato PI-I. Northern blot analyses indicated that healthy tobacco leaf contains only minor amounts of PI-I mRNA, and that the inhibitor genes are induced by TMV infection, salicylate treatment, ethephon spraying, UV light irradiation and wounding. The results indicate that the tobacco PI-I genes are coordinately expressed with the genes for the basic pathogenesis-related proteins. Contrary to PI-I genes of tomato and potato, wound induction of the tobacco genes occurs only locally; the upper, unwounded leaves do not show any wound-induced PI-I gene expression. |
| Databáze: | OpenAIRE |
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