Cyclin D1 Immunoreactivity in Meningiomas
| Autor: | Tatjana Marinkovic, Milan B. Jovanovic, Slavisa Djuricic, Ivana I. Berisavac, Iva Berisavac, Sanja Milenkovic |
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| Rok vydání: | 2008 |
| Předmět: |
Pathology
medicine.medical_specialty Meningioma Cellular and Molecular Neuroscience Cyclin D1 Proliferating Cell Nuclear Antigen Biomarkers Tumor Meningeal Neoplasms otorhinolaryngologic diseases medicine Humans neoplasms Cell Proliferation biology Cell growth Cell Biology General Medicine Cell cycle medicine.disease Immunohistochemistry nervous system diseases Proliferating cell nuclear antigen Ki-67 Antigen biology.protein Cancer research Antibody Immunostaining |
| Zdroj: | Cellular and Molecular Neurobiology. 28:907-913 |
| ISSN: | 1573-6830 0272-4340 |
| DOI: | 10.1007/s10571-008-9278-x |
| Popis: | Objective Cyclin D1 is an important nuclear protein required for progression of cells through the G1 phase of the cell cycle. The proliferative potential of meningiomas has been studied using various proliferative markers. However, there have been only few published studies evaluating Cyclin D1 immunoreactivity in meningiomas. Purpose of the study The aim of our study was to analyze the Cyclin D1 expression in meningiomas and correlate it both with proliferation markers Ki67 and PCNA, and with meningiomas of WHO grade. Material and methods We evaluated immunoreactivity for proliferative markers (Cyclin D1, Ki-67, and PCNA) in a consecutive series of 64 meningioma samples obtained from patients who underwent surgical resection because of cerebral or spinal meningiomas. Immunohistochemical staining with Ki-67, PCNA, and Cyclin D1 was performed using the microwave processing procedure and LSAB+ methodology. The number of positive cells for each antibody has been determined and shown in percentage in relation to 1000 counted cells. Results All meningioma samples showed immunostaining for Ki-67, PCNA, and Cyclin D1 antibodies. The Cyclin D1 scores exhibited a close correlation with Ki-67 and PCNA immunostaining (P |
| Databáze: | OpenAIRE |
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