Virological and Molecular Characterisation of a New B Lymphoid Cell Line, Established from an AIDS Patient with Primary Effusion Lymphoma, Harbouring Both KSHV/HHV8 and EBV Viruses

Autor: Cadranel J, Elisabeth Macintyre, Lacoste, Eric Delabesse, Bestett G, Antoine M, Françoise Valensi, Antoine Gessain, Judde Jg
Přispěvatelé: Epidémiologie et Physiopathologie des Virus Oncogènes (EPVO (UMR_3569 / U-Pasteur_3)), Institut Pasteur [Paris] (IP)-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), CHU Tenon [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Service d'hématologie [CHU Necker], CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris]-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Service de pneumologie et réanimation [CHU Tenon], Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-CHU Tenon [APHP], Service d'anatomie pathologique [CHU Tenon], CHU Necker - Enfants Malades [AP-HP]-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7)-Institut Pasteur [Paris], Service de Pneumologie - Oncologie Thoracique - Maladies Pulmonaires Rares [CHU Tenon], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Tenon [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)
Rok vydání: 2000
Předmět:
Male
Epstein-Barr Virus Infections
Herpesvirus 4
Human
Cancer Research
MESH: Tumor Cells
Cultured/virology
viruses
clonality
MESH: Lymphoma
B-Cell/pathology
MESH: Epstein-Barr Virus Infections/virology
CD38
Virus Replication
MESH: Gene Expression Regulation
Viral
0302 clinical medicine
MESH: Tumor Cells
Cultured/metabolism
MESH: Reverse Transcriptase Polymerase Chain Reaction
hemic and lymphatic diseases
MESH: B-Lymphocytes/metabolism
MESH: B-Lymphocytes/virology
Tumor Cells
Cultured
MESH: DNA
Viral/genetics
Gene Rearrangement
B-Lymphocyte
Lymphoma
AIDS-Related
MESH: DNA
Viral/isolation & purification
B-Lymphocytes
MESH: Pleural Effusion
Malignant/virology
0303 health sciences
Genes
Immunoglobulin
Reverse Transcriptase Polymerase Chain Reaction
MESH: Gene Rearrangement
B-Lymphocyte
MESH: Herpesviridae Infections/pathology
MESH: Herpesvirus 4
Human/physiology
MESH: Herpesviridae Infections/virology
virus diseases
DNA
Neoplasm
Herpesviridae Infections
MESH: Gene Expression Regulation
Neoplastic
Hematology
MESH: Neoplastic Stem Cells/metabolism
MESH: Lymphoma
B-Cell/virology
3. Good health
Gene Expression Regulation
Neoplastic
Oncology
Lytic cycle
MESH: B-Lymphocytes/pathology
MESH: Cell Transformation
Viral
030220 oncology & carcinogenesis
Herpesvirus 8
Human
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
Neoplastic Stem Cells
MESH: Epstein-Barr Virus Infections/pathology
Primary effusion lymphoma
MESH: Pleural Effusion
Malignant/pathology
Adult
Gene Expression Regulation
Viral
Lymphoma
B-Cell
MESH: Herpesvirus 8
Human/physiology
MESH: Immunophenotyping
MESH: Epstein-Barr Virus Infections/complications
KSHV/HHV8
Biology
MESH: Neoplastic Stem Cells/pathology
Virus
Immunophenotyping
Open Reading Frames
03 medical and health sciences
MESH: Tumor Virus Infections/pathology
MESH: Tumor Virus Infections/virology
Antigen
EBV
medicine
Humans
MESH: Tumor Virus Infections/complications
MESH: DNA
Neoplasm/genetics
primary effusion lymphoma
030304 developmental biology
MESH: Herpesvirus 4
Human/isolation & purification
MESH: Humans
MESH: Virus Replication
MESH: Adult
MESH: Lymphoma
AIDS-Related/virology
MESH: Herpesviridae Infections/complications
Gene rearrangement
MESH: Open Reading Frames
Cell Transformation
Viral
medicine.disease
Virology
MESH: Herpesvirus 8
Human/isolation & purification
MESH: Male
Pleural Effusion
Malignant
Lymphoma
MESH: Genes
Immunoglobulin
Tumor Virus Infections
DNA
Viral
MESH: Neoplastic Stem Cells/virology
MESH: Lymphoma
AIDS-Related/pathology
Immunoglobulin heavy chain
Zdroj: Leukemia & lymphoma
Leukemia & lymphoma, 2000, 38 (3-4), pp.401-409. ⟨10.3109/10428190009087032⟩
Leukemia & lymphoma, Taylor & Francis, 2000, 38 (3-4), pp.401-409. ⟨10.3109/10428190009087032⟩
ISSN: 1029-2403
1042-8194
DOI: 10.3109/10428190009087032
Popis: International audience; We report here a new case of primary effusion lymphoma (PEL), occurring in a French homosexual HIV-1 infected male with a pericardial, pleural and mesenteric tumour dissemination, and the establishment from his pleural effusion of a new cell line, Cra-BCBL, dually infected by EBV and KSHV/HHV8. Cra-BCBL cells are of B-cell origin as judged by their clonal immunoglobulin heavy chain (IgH) gene rearrangement, identical to that of the parental tumour. Both the cell line and the lymphoma cells expressed CD38 and CD45 antigens but no classical B-cell or T-cell lineage-restricted antigens. Cra-BCBL harbours a type I EBV virus, expressing a latency type II. Expression of KSHV/HHV8 ORF72 and ORF75 was detected by RT/PCR. In addition, KSHV lytic replication could be induced by treatment by n-butyrate. An equivalent and high copy number of KSHV genomes (20 to 200 copies by cell) was detected both in the primary tumour cells and in the cell line. Southern blot (SB) analysis of EBV terminal repeats (TR) displayed the same unique band in the cell line DNA and in the original tumour cells, consistent with a monoclonal infection of EBV. Furthermore, SB analysis of KSHV/HHV8 TR revealed the same hybridisation pattern between Cra-BCBL and the effusion cells, with a common band at around 30-40 kb corresponding to the fused termini of the viral episomes and a 5 Kb rearranged fragment. The new cell line characterised here could be a useful model to study interactions between two human herpes viruses and their contribution to lymphomagenesis.
Databáze: OpenAIRE
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