Detection of low-molecular-weight proteins in urine by dipsticks
| Autor: | Larry Clark, Todd K. Cast, Michael J. Pugia, James A. Profitt, David J. Newman, Lucia D'Mello, John A. Lott |
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| Rok vydání: | 2002 |
| Předmět: |
Quality Control
Urinalysis Pyrogallol red Clinical Biochemistry Urine urologic and male genital diseases Sensitivity and Specificity Biochemistry parasitic diseases medicine Humans Coloring Agents Reagent Strips Chromatography Reagent strip medicine.diagnostic_test Chemistry Biochemistry (medical) Proteins Reproducibility of Results General Medicine Dipstick Molecular Weight Proteinuria Chemical sensitivity Creatinine |
| Zdroj: | Clinica Chimica Acta. 326:177-183 |
| ISSN: | 0009-8981 |
| DOI: | 10.1016/s0009-8981(02)00304-2 |
| Popis: | Background : Testing of urines with dipsticks for proteinuria, glycosuria, etc., is common practice. A deficiency with currently available dipsticks is their lack of chemical sensitivity and underestimation of low-molecular-weight proteins such as light chains. Methods : We experimented with a number of dyes that gave an easily recognized color change on dipsticks for various low-molecular-weight proteins such as α-1-glycoprotein, α-1- and β-2-microglobulin, and kappa and lambda light chains. We were successful in formulating a dye for impregnating dipsticks that gave a color change with low-molecular-weight proteins. Results : Most dipsticks will measure proteins down to about 1 g/l. Our composite of two dyes (described here as the “TPR” dipsticks) gave reproducible results for protein concentrations of ≥300 mg/l, and detected low-molecular proteins. The TPR reagent is resistant to interferences from many compounds; also, the protein results are not altered in a given urine at a pH between 5 and 8. Conclusions : We have developed a dipstick that detects low-molecular-weight proteins. The dipsticks are easy to use and are suitable for outpatient or point-of-care testing. The precision of the dipsticks is satisfactory and is only marginally lower than quantitative spectrophotometric methods using pyrogallol red (PYR). |
| Databáze: | OpenAIRE |
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