Transcriptional Analysis of Drought-Induced Genes in the Roots of a Tolerant Genotype of the Common Bean (Phaseolus vulgaris L.)

Autor: Ana Luiza Ahern Beraldo, Siu Mui Tsai, Márcio José da Silva, Gustavo Henrique Recchia, Danielle Gregorio Gomes Caldas
Jazyk: angličtina
Rok vydání: 2013
Předmět:
Time Factors
Transcription
Genetic
Plant Roots
lcsh:Chemistry
Soil
Gene Expression Regulation
Plant
Gene expression
Genotype
Cluster Analysis
lcsh:QH301-705.5
Spectroscopy
Expressed Sequence Tags
Phaseolus
Genetics
Expressed sequence tag
Reverse Transcriptase Polymerase Chain Reaction
drought stress
food and beverages
General Medicine
Adaptation
Physiological
Droughts
Computer Science Applications
Subtractive Hybridization Techniques
Biology
Genes
Plant
Article
Catalysis
Inorganic Chemistry
Botany
RNA
Messenger
Physical and Theoretical Chemistry
Phaseolus vulgaris L
root
suppressive subtractive hybridization library
gene expression
Molecular Biology
Gene
Gene Expression Profiling
Organic Chemistry
Reproducibility of Results
Water
Humidity
biology.organism_classification
Gene expression profiling
lcsh:Biology (General)
lcsh:QD1-999
Suppression subtractive hybridization
Software
Transcription Factors
Zdroj: International Journal of Molecular Sciences, Vol 14, Iss 4, Pp 7155-7179 (2013)
International Journal of Molecular Sciences; Volume 14; Issue 4; Pages: 7155-7179
International Journal of Molecular Sciences
ISSN: 1422-0067
Popis: In Brazil, common bean (Phaseolus vulgaris L.) productivity is severely affected by drought stress due to low technology cultivation systems. Our purpose was to identify differentially expressed genes in roots of a genotype tolerant to water deficit (BAT 477) when submitted to an interruption of irrigation during its development. A SSH library was constructed taking as “driver” the genotype Carioca 80SH (susceptible to drought). After clustering and data mining, 1572 valid reads were obtained, resulting in 1120 ESTs (expressed sequence tags). We found sequences for transcription factors, carbohydrates metabolism, proline-rich proteins, aquaporins, chaperones and ubiquitins, all of them organized according to their biological processes. Our suppressive subtractive hybridization (SSH) library was validated through RT-qPCR experiment by assessing the expression patterns of 10 selected genes in both genotypes under stressed and control conditions. Finally, the expression patterns of 31 ESTs, putatively related to drought responses, were analyzed in a time-course experiment. Our results confirmed that such genes are more expressed in the tolerant genotype during stress; however, they are not exclusive, since different levels of these transcripts were also detected in the susceptible genotype. In addition, we observed a fluctuation in gene regulation over time for both the genotypes, which seem to adopt and adapt different strategies in order to develop tolerance against this stress.
Databáze: OpenAIRE
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