Copper toxicity in expanding leaves of Phaseolus vulgaris L.: antioxidant enzyme response and nutrient element uptake
| Autor: | Anja Geitmann, Ezzeddine El Ferjani, Hager Jouili, Houda Bouazizi |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Antioxidant
Health Toxicology and Mutagenesis medicine.medical_treatment Iron Antioxidants chemistry.chemical_compound medicine Soil Pollutants Food science Plant Physiological Phenomena Phaseolus Glutathione Peroxidase Chlorosis biology Chemistry Copper toxicity Public Health Environmental and Occupational Health General Medicine Malondialdehyde medicine.disease Catalase Pollution Enzyme assay Plant Leaves Zinc Biochemistry Toxicity biology.protein Potassium Lipid Peroxidation Plant nutrition Copper |
| Zdroj: | Ecotoxicology and environmental safety. 73(6) |
| ISSN: | 1090-2414 |
| Popis: | Bioaccumulation and toxicity of copper (CuSO4) were assessed in expanding leaves of 14-day-old bean seedlings. CuSO4 was administrated in the growth medium for three days and changes in the activities of the antioxidant enzymes guaiacol peroxidase (GPX) and catalase (CAT), and in the H2O2 production and mineral element contents were measured. Copper accumulated in exposed plants caused severe symptoms such as chlorosis and necrosis as well as a dramatic reduction in dry weight production. Simultaneously, concentrations of iron, zinc and potassium were reduced significantly suggesting that a change in nutrient homeostasis may be responsible for the observed symptoms. Contrary to mature tissues, the expanding leaves did not display significant oxidative stress, since malondialdehyde (MDA) content was unchanged, the activities of GPX and CAT were lowered or unaltered, and endogenous H2O2 only increased at high copper concentrations. Our results suggest that while excess copper slightly alters the activity of the antioxidative enzyme system in young expanding leaves of bean plants, it exerts its toxicity primarily through causing a disturbance in the nutrient balance. |
| Databáze: | OpenAIRE |
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