Accumulation and elimination profiles of paralytic shellfish poison in the short-necked clam Tapes japonica fed with the toxic dinoflagellate Gymnodinium catenatum
| Autor: | Mohamad Samsur, Takefumi Sagara, Tamao Noguchi, Yasunaga Yamaguchi, Tomohiro Takatani, Osamu Arakawa |
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| Rok vydání: | 2007 |
| Předmět: |
Carbamate
animal structures Chromatography biology Toxin medicine.medical_treatment Decarbamoylsaxitoxin Dinoflagellate General Medicine biology.organism_classification medicine.disease_cause Gymnodinium catenatum Japonica Bivalvia chemistry.chemical_compound chemistry medicine Dinoflagellida Animals Marine Toxins Derivatization Feces Chromatography High Pressure Liquid |
| Zdroj: | Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan. 48(1) |
| ISSN: | 0015-6426 |
| Popis: | The paralytic shellfish poison (PSP)-producing dinoflagellate Gymnodinium catenatum (Gc) was fed to the short-necked clam Tapes japonica, and the accumulation, transformation and elimination profiles of PSP were investigated by means of high-performance liquid chromatography with postcolumn fluorescence derivatization (HPLC-FLD). The short-necked clams ingested most of the Gc cells (4 x 10(6) cells) supplied as a bolus at the beginning of the experiment, and accumulated a maximal amount of toxin (181 nmol/10 clams) after 12 hr. The rate of toxin accumulation at that time was 16%, which rapidly decreased thereafter. During the rearing period, a variation in toxin composition, derived presumably from the transformation of toxin analogues in the clams, was observed, including a reversal of the ratio of C2 to C1, and the appearance of carbamate (gonyautoxin (GTX) 2, 3) and decarbamoyl (dc) derivatives (decarbamoylsaxitoxin (dcSTX) and dcGTX2, 3), which were undetectable in Gc cells. The total amount of toxin contained in clams and residue (remaining Gc cells and/or excrement in the rearing tank) gradually declined, and only about 1% of the supplied toxin was detected at the end of the experiment. |
| Databáze: | OpenAIRE |
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