A Biomimetic Phosphatidylcholine-Terminated Monolayer Greatly Improves the In Vivo Performance of Electrochemical Aptamer-Based Sensors
| Autor: | Hui Li, Philippe Dauphin-Ducharme, Jacob Somerson, Claire H. Tran, Shaoguang Li, Christina B. Shin, Philip A. Vieira, Netzahualcóyotl Arroyo-Currás, Tod E. Kippin, Kevin W. Plaxco |
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| Rok vydání: | 2017 |
| Předmět: |
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Aptamer aptamers Nanotechnology 02 engineering and technology Biosensing Techniques Electrochemistry 010402 general chemistry Living body 01 natural sciences Article Catalysis biomimetic surfaces chemistry.chemical_compound In vivo Biomimetics membrane monolayers Phosphatidylcholine Monolayer Animals in vivo measurements Cell Membrane Organic Chemistry electrochemical sensors General Chemistry General Medicine Electrochemical Techniques Aptamers Nucleotide 021001 nanoscience & nanotechnology 3. Good health 0104 chemical sciences Good Health and Well Being chemistry Physical Barrier Chemical Sciences Phosphatidylcholines 0210 nano-technology Nucleotide Blood Chemical Analysis Algorithms Biotechnology |
| Zdroj: | Angewandte Chemie (International ed. in English), vol 56, iss 26 Angew. Chem. Int. Ed. |
| Popis: | The real-time monitoring of specific analytes in situ in the living body would greatly advance our understanding of physiology and the development of personalized medicine. Because they are continuous (wash-free and reagentless) and are able to work in complex media (e.g., undiluted serum), electrochemical aptamer-based (E-AB) sensors are promising candidates to fill this role. E-AB sensors suffer, however, from often-severe baseline drift when deployed in undiluted whole blood either in vitro or in vivo. We demonstrate that cell-membrane-mimicking phosphatidylcholine (PC)-terminated monolayers improve the performance of E-AB sensors, reducing the baseline drift from around 70 % to just a few percent after several hours in flowing whole blood in vitro. With this improvement comes the ability to deploy E-AB sensors directly in situ in the veins of live animals, achieving micromolar precision over many hours without the use of physical barriers or active drift-correction algorithms. |
| Databáze: | OpenAIRE |
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