Campylobacter culture fails to correctly detect Campylobacter in 30% of positive patient stool specimens compared to non-cultural methods

Autor: Blake W. Buchan, Janice E. Buss, David Craft, Michelle Cresse, Steve Young, Susan Doyle
Rok vydání: 2019
Předmět:
Male
0301 basic medicine
Culture
medicine.disease_cause
Gastroenterology
Feces
0302 clinical medicine
Medical microbiology
Campylobacter spp
Campylobacter Infections
Prospective Studies
030212 general & internal medicine
Child
Aged
80 and over
Immunoassay
medicine.diagnostic_test
biology
Campylobacter
General Medicine
Middle Aged
Infectious Diseases
Molecular Diagnostic Techniques
Child
Preschool
Female
Original Article
Campylobacter upsaliensis
Adult
Microbiology (medical)
medicine.medical_specialty
Adolescent
030106 microbiology
Campylobacteriosis
Sensitivity and Specificity
Young Adult
03 medical and health sciences
Internal medicine
Multiplex polymerase chain reaction
medicine
Humans
Aged
Composite reference method
Bacteriological Techniques
business.industry
Infant
16S ribosomal RNA
biology.organism_classification
medicine.disease
business
Zdroj: European Journal of Clinical Microbiology & Infectious Diseases
ISSN: 1435-4373
0934-9723
Popis: Campylobacter diagnosis is hampered because many laboratories continue to use traditional stool culture, which is slow and suffers false-negative results. This large multi-site study used a composite reference method consisting of a new FDA-cleared immunoassay and four molecular techniques to compare to culture. Prospectively collected patient fecal specimens (1552) were first preliminarily categorized as positive or negative by traditional culture. All specimens were also tested by EIA, and any EIA-positive or culture-discrepant results were further characterized by 16S rRNA qPCR, eight species-specific PCR assays, bidirectional sequencing, and an FDA-cleared multiplex PCR panel. The five non-culture methods showed complete agreement on all positive and discrepant specimens which were then assigned as true-positive or true-negative specimens. Among 47 true-positive specimens, culture incorrectly identified 13 (28%) as negative, and 1 true-negative specimen as positive, for a sensitivity of 72.3%. Unexpectedly, among the true-positive specimens, 4 (8%) were the pathogenic species C. upsaliensis. Culture had a 30% false result rate compared to immunoassay and molecular methods. More accurate results lead to better diagnosis and treatment of suspected campylobacteriosis.
Databáze: OpenAIRE
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