Enterobacter cloacae administration induces hepatic damage and subcutaneous fat accumulation in high-fat diet fed mice
| Autor: | Pentti Huovinen, Eveliina Munukka, Raine Toivonen, Heikki Kainulainen, Maija Hollmén, Anniina Keskitalo, Sirpa Jalkanen, Satu Pekkala |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
rasvahapot
Pathology and Laboratory Medicine rasvat (orgaaniset yhdisteet) ruokavaliot Biochemistry Mice Animal Cells Fibrosis lcsh:Science Immune Response Connective Tissue Cells Chemical Reactions ta3141 ta3142 Lipids 3. Good health Physical sciences Adiponectin Cellular Types medicine.medical_specialty fats Lipolysis Immunology Subcutaneous Fat rasvakudokset Monomers (Chemistry) glycerol Diet High-Fat ta3111 03 medical and health sciences Signs and Symptoms Enterobacter cloacae Polymer chemistry lcsh:R Biology and Life Sciences Hypertrophy medicine.disease Receptor Insulin Mice Inbred C57BL Biological Tissue 030104 developmental biology Endocrinology lihavuus lcsh:Q Glycerol 0301 basic medicine Physiology Liver cytology suolistomikrobisto lcsh:Medicine Adipose tissue Gut flora Medicine and Health Sciences Adipocytes enterobakteerit 2. Zero hunger rasvat Multidisciplinary gastrointestinal microbiota tulehdus biology Hydrolysis adipose tissue Chemistry Physiological Parameters Liver Connective Tissue embryonic structures Female Anatomy medicine.symptom Research Article animal structures adipocytes Inflammation Insulin resistance Enterobacteriaceae Diagnostic Medicine Internal medicine medicine Animals Obesity Triglycerides Nutrition urogenital system business.industry Body Weight Cell Biology biology.organism_classification Diet Toll-Like Receptor 5 Gene Expression Regulation inflammation lipolysis diet business |
| Zdroj: | PLoS ONE PLoS ONE, Vol 13, Iss 5, p e0198262 (2018) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0198262 |
| Popis: | Accumulating evidence indicates that gut microbiota plays a significant role in obesity, insulin resistance and associated liver disorders. Family Enterobacteriaceae and especially Enterobacter cloacae strain B29 have been previously linked to obesity and hepatic damage. The underlying mechanisms, however, remain unclear. Therefore, we comprehensively examined the effects of E. cloacae subsp. cloacae (ATCC® 13047™) administration on host metabolism of mice fed with high-fat diet (HFD). C57BL/6N mice were randomly divided into HFD control, chow control, and E. cloacae treatment groups. The E. cloacae treatment group received live bacterial cells in PBS intragastrically twice a week, every other week for 13 weeks. Both control groups received PBS intragastrically. After the 13- week treatment period, the mice were sacrificed for gene and protein expression and functional analyses. Our results show that E. cloacae administration increased subcutaneous fat mass and the relative proportion of hypertrophic adipocytes. Both subcutaneous and visceral fat had signs of decreased insulin signaling and elevated lipolysis that was reflected in higher serum glycerol levels. In addition, E. cloacae -treated mice had significantly higher hepatic AST and AST/ALT ratio, and their liver histology indicated fibrosis, demonstrating that E. cloacae subsp. cloacae administration promotes hepatic damage in HFD fed mice. peerReviewed |
| Databáze: | OpenAIRE |
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