Mechanical segregation and capturing of clonal circulating plasma cells in multiple myeloma using micropillar-integrated microfluidic device
| Autor: | Liangcheng Xu, Chen Wang, Jaewon Park, Lidan You, Lina Hu, Weicong Lin, Wenqi He, Dongfang Ouyang, Yonghua Li |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Pathology
medicine.medical_specialty Biomedical Engineering 02 engineering and technology Immunoglobulin light chain 01 natural sciences Colloid and Surface Chemistry medicine Distribution (pharmacology) General Materials Science Multiple myeloma Fluid Flow and Transfer Processes business.industry 010401 analytical chemistry Cancer Gold standard (test) 021001 nanoscience & nanotechnology Condensed Matter Physics medicine.disease Minimal residual disease 0104 chemical sciences 3. Good health medicine.anatomical_structure Monoclonal Bone marrow 0210 nano-technology business Regular Articles |
| Zdroj: | Biomicrofluidics |
| ISSN: | 1932-1058 |
| Popis: | Multiple myeloma (MM), the disorder of plasma cells, is the second most common type of hematological cancer and is responsible for approximately 20% of deaths from hematological malignancies. The current gold standard for MM diagnosis includes invasive bone marrow aspiration. However, it lacks the sensitivity to detect minimal residual disease, and the nonuniform distribution of clonal plasma cells (CPCs) within bone marrow also often results in inaccurate reporting. Serum and urine assessment of monoclonal proteins, such as Kappa light chains, is another commonly used approach for MM diagnosis. Although it is noninvasive, the level of paraprotein elevation is still too low for detecting minimal residual disease and nonsecretive MM. Circulating CPCs (cCPCs) have been reported to be present in the peripheral blood of MM patients, and high levels of cCPCs were shown to correlate with poor survival. This suggests a potential noninvasive approach for MM disease progress monitoring and prognosis. In this study, we developed a mechanical property-based microfluidic platform to capture cCPCs. Using human myeloma cancer cell lines spiked in healthy donor blood, the microfluidic platform demonstrates high enrichment ratio (>500) and sufficient capture efficiency (40%-55%). Patient samples were also assessed to investigate the diagnostic potential of cCPCs for MM by correlating with the levels of Kappa light chains in patients. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|