Strain differences in antioxidants in rat models of cardiovascular disease exposed to ozone

Autor:	J. E. Schmid, Gary E. Hatch, Kay M. Crissman, Allen D. Ledbetter, William O. Ward, Judy E. Richards, Urmila P. Kodavanti, Mette C. Schladweiler
Rok vydání:	2015
Předmět:	medicine.medical_specialty Antioxidant Health Toxicology and Mutagenesis medicine.medical_treatment Toxicology medicine.disease_cause Thiobarbituric Acid Reactive Substances Antioxidants Gene Expression Regulation Enzymologic Superoxide dismutase chemistry.chemical_compound Ozone Internal medicine medicine Animals Lung Cardiopulmonary disease Aconitate Hydratase Air Pollutants Inhalation Exposure medicine.diagnostic_test biology Chemistry Superoxide Dismutase Rats Inbred Strains respiratory system medicine.disease respiratory tract diseases Rats Disease Models Animal Endocrinology Bronchoalveolar lavage Cardiovascular Diseases Toxicity Immunology biology.protein Uric acid Metabolic syndrome Transcriptome Bronchoalveolar Lavage Fluid Oxidative stress
Zdroj:	Inhalation toxicology. 27
ISSN:	1091-7691
Popis:	We examined the hypothesis that antioxidant substances and enzymes in lung, heart and in bronchoalveolar lavage fluid (BALF) are altered in response to O3 in cardiovascular disease and/or metabolic syndrome (CVD)-prone rat models. CVD strains [spontaneously hypertensive (SH), SH stroke-prone (SHSP), SHHF/Mcc heart failure obese (SHHF), insulin-resistant JCR:LA-cp obese (JCR) and Fawn-Hooded hypertensive (FHH)] were compared with normal strains [Wistar, Sprague-Dawley (SD) and Wistar Kyoto (WKY)]. Total glutathione (GSH + GSSG or GSx), reduced ascorbate (AH2), uric acid (UA) and antioxidant enzymes were determined in lung, heart and BALF immediately (0 h) or 20-h post 4-h nose-only exposure to 0.0, 0.25, 0.5 and 1.0 ppm O3. Basal- and O3-induced antioxidant substances in tissues varied widely among strains. Wistar rats had a robust O3-induced increase in GSx and AH2 in the lung. Two CVD strains (JCR and SHHF) had high basal levels of AH2 and GSx in BALF as well as high basal lung UA. Across all strains, high BALF GSx was only observed when high BALF AH2 was present. CVD rats tended to respond less to O3 than normal. High-basal BALF AH2 levels were associated with decreased O3 toxicity. In summary, large differences were observed between both normal and CVD rat strains in low-molecular weight antioxidant concentrations in lung, BALF and heart tissue. Wistar (normal) and JCR and SHHF (CVD) rats appeared to stand out as peculiar in terms of basal- or O3-induced changes. Results elucidate interactions among antioxidants and air pollutants that could enhance understanding of cardiopulmonary disease.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0bd3078c78b877c59f824575b635d98e https://pubmed.ncbi.nlm.nih.gov/26667331 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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