Alanine-Threonine Polymorphism of Helicobacter pylori RpoB Is Correlated with Differential Induction of Interleukin-8 in MKN45 Cells
| Autor: | Kwang Ho Rhee, David Y. Graham, Hyun Chae Jung, Chang Young Lim, Myung Je Cho, So Yon Woo, Woo Kon Lee, Yeo Jun Yun, Keun Hwa Lee, Yoon Hoh Kook, Yoshio Yamaoka, Kwan Soo Ko, Bum Joon Kim |
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| Rok vydání: | 2004 |
| Předmět: |
DNA
Bacterial Threonine Microbiology (medical) Population Polymerase Chain Reaction Cell Line Microbiology Polymorphism (computer science) polycyclic compounds Animals Interleukin 8 education Phylogeny DNA Primers Alanine Genetics Genetic diversity education.field_of_study Base Sequence Geography Helicobacter pylori biology Interleukin-8 Bacteriology DNA-Directed RNA Polymerases bacterial infections and mycoses biology.organism_classification rpoB Coculture Techniques Amino Acid Substitution |
| Zdroj: | Journal of Clinical Microbiology. 42:3518-3524 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.42.8.3518-3524.2004 |
| Popis: | Geographical differences in the genetic diversity of Helicobacter pylori isolates were examined by analyzing rpoB sequences. An extremely high level of allelic diversity among H. pylori strains was found. The rpoB sequences of Asian and non-Asian (North and South American, European, and South African) strains were found to differ. An amino acid polymorphism (alanine and threonine RpoB types) was found at the 497th residue by deduced amino acid analysis. RpoB with a threonine residue (RpoB Thr ) was uniquely present in East Asian countries, and two-thirds of the H. pylori isolate population in this region was RpoB Thr ; however, this type was rare or absent in Western countries, where RpoB Ala predominated. RpoB Thr strains induced a much larger amount of interleukin-8, a chemokine that plays an important role in chronic inflammation, than RpoB Ala strains in cultured MKN45 cells. |
| Databáze: | OpenAIRE |
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