In vitro models of the blood–brain barrier: An overview of commonly used brain endothelial cell culture models and guidelines for their use
| Autor: | Carola Förster, Babette B. Weksler, Mária A. Deli, Pierre Olivier Couraud, Birger Brodin, H.-J. Galla, N. Joan Abbott, Elodie Vandenhaute, Hans Christian Cederberg Helms, Malgorzata Burek, Matthew J. Stebbins, Roméo Cecchelli, Eric V. Shusta, Ignacio A. Romero |
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| Přispěvatelé: | University of Copenhagen = Københavns Universitet (KU), King‘s College London, University of Würzburg, Laboratoire de la Barrière Hémato-Encéphalique (LBHE), Université d'Artois (UA), Institut Cochin (IC UM3 (UMR 8104 / U1016)), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institute of Biophysics, Biological Research Centre [Budapest] (BRC), Hungarian Academy of Sciences (MTA)-Hungarian Academy of Sciences (MTA), University of Muenster, Department of Life, Health and Chemical Sciences, The Open University [Milton Keynes] (OU), University of Wisconsin-Madison, Division of Hematology and Oncology, Weill Medical College of Cornell University [New York], Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Justin-Labonne, Virginie, University of Copenhagen = Københavns Universitet (UCPH) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Endothelium endothelium [SDV]Life Sciences [q-bio] Drug delivery to the brain Guidelines as Topic Biology Blood–brain barrier Models Biological pericytes 03 medical and health sciences 0302 clinical medicine stem cells Parenchyma medicine Humans Review Articles Neuroinflammation Cells Cultured astrocytes Brain Endothelial Cells 3. Good health Endothelial stem cell [SDV] Life Sciences [q-bio] 030104 developmental biology medicine.anatomical_structure Neurology Cell culture Blood-Brain Barrier Neurology (clinical) Endothelium Vascular Stem cell Cardiology and Cardiovascular Medicine Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Journal of Cerebral Blood Flow and Metabolism Journal of Cerebral Blood Flow and Metabolism, Nature Publishing Group, 2016, 36 (5), pp.862-890. ⟨10.1177/0271678X16630991⟩ Journal of Cerebral Blood Flow and Metabolism, 2016, 36 (5), pp.862-890. ⟨10.1177/0271678X16630991⟩ |
| ISSN: | 0271-678X 1559-7016 |
| DOI: | 10.1177/0271678X16630991⟩ |
| Popis: | International audience; The endothelial cells lining the brain capillaries separate the blood from the brain parenchyma. The endothelial monolayer of the brain capillaries serves both as a crucial interface for exchange of nutrients, gases, and metabolites between blood and brain, and as a barrier for neurotoxic components of plasma and xenobiotics. This "blood-brain barrier" function is a major hindrance for drug uptake into the brain parenchyma. Cell culture models, based on either primary cells or immortalized brain endothelial cell lines, have been developed, in order to facilitate in vitro studies of drug transport to the brain and studies of endothelial cell biology and pathophysiology. In this review, we aim to give an overview of established in vitro blood-brain barrier models with a focus on their validation regarding a set of well-established blood-brain barrier characteristics. As an ideal cell culture model of the blood-brain barrier is yet to be developed, we also aim to give an overview of the advantages and drawbacks of the different models described. |
| Databáze: | OpenAIRE |
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