Distinct Proteins in Protein Corona of Nanoparticles Represent a Promising Venue for Endogenous Targeting – Part I: In vitro Release and Intracellular Uptake Perspective

Autor: Aliaa Nabil ElMeshad, Ulrike Breitinger, Iman Gomaa, Mahmoud H. Abdel-Kader, Hans-Georg Breitinger, Aya Ahmed Sebak, Mahmoud Hussien Farag
Jazyk: angličtina
Rok vydání: 2020
Předmět:
inorganic chemicals
active targeting
Biophysics
Intracellular Space
Pharmaceutical Science
Bioengineering
Protein Corona
Peptide
02 engineering and technology
010402 general chemistry
01 natural sciences
intracellular uptake
Peptides
Cyclic
Biomaterials
chemistry.chemical_compound
protein corona
Polylactic Acid-Polyglycolic Acid Copolymer
Drug Discovery
mental disorders
melanoma
Humans
Bradford protein assay
health care economics and organizations
Original Research
chemistry.chemical_classification
endogenous targeting
Drug Carriers
Chemistry
Organic Chemistry
technology
industry
and agriculture
Biological Transport
General Medicine
respiratory system
021001 nanoscience & nanotechnology
passive targeting
Blood proteins
In vitro
0104 chemical sciences
PLGA
Drug Liberation
Nanoparticles
Nanocarriers
0210 nano-technology
Intracellular
Zdroj: International Journal of Nanomedicine
ISSN: 1178-2013
1176-9114
Popis: Introduction Protein corona (PC) deposition on nanoparticles (NPs) in biological systems contributes to a great extent to NPs' fates; their targeting potential, the interaction with different biological systems and the subsequent functions. PC - when properly tuned - can serve as a potential avenue for optimization of NPs' use in cancer therapy. Methods Poly-lactic co-glycolic acid (PLGA)-based NPs exhibiting different physicochemical properties were fabricated and characterized. The PC makeup of these NPs were qualitatively and quantitatively analyzed by Western blot and Bradford assay, respectively. The effect of PC on the release of NPs' cargos and the intracellular uptake into B16F10 melanoma cells has been studied. Results The composition of NPs (polymeric PLGA NPs vs lipid-polymer hybrid NPs) and the conjugation of an active targeting ligand (cRGDyk peptide) represented the major determinants of the PC makeup of NPs. The in vitro release of the loaded cargos from the NPs depended on the PC and the presence of serum proteins in the release medium. Higher cumulative release has been recorded in the presence of proteins in the case of peptide conjugated NPs, cNPs, while the unconjugated formulations, uNPs, showed an opposite pattern. NPs intracellular uptake studies revealed important roles of distinct serum and cellular proteins on the extent of NPs' accumulation in melanoma cells. For example, the abundance of vitronectin (VN) protein from serum has been positively related to the intracellular accumulation of the NPs. Conclusion Careful engineering of nanocarriers can modulate the recruitment of some proteins suggesting a potential use for achieving endogenous targeting to overcome the current limitations of targeted delivery of chemotherapeutic agents.
Databáze: OpenAIRE
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