Thermomechanical Response of a Representative Porin for Biomimetics
| Autor: | Eric A. Perpète, Anna Puiggalí-Jou, Maximilien Lopes-Rodrigues, Catherine Michaux, Luis J. del Valle, Carlos Alemán, Didac Martí-Balleste |
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| Přispěvatelé: | Universitat Politècnica de Catalunya. Departament d'Enginyeria Química, Universitat Politècnica de Catalunya. IMEM-BRT- Innovation in Materials and Molecular Engineering - Biomaterials for Regenerative Therapies, Universitat Politècnica de Catalunya. PSEP - Polimers Sintètics: Estructura i Propietats. Polimers Biodegradables., Universitat Politècnica de Catalunya. PSEP - Polimers Sintètics: Estructura i Propietats. Polimers Biodegradables |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Materials science
Thermal properties General Chemical Engineering Mechanical properties 02 engineering and technology Molecular dynamics 010402 general chemistry 01 natural sciences Article lcsh:Chemistry chemistry.chemical_compound Enginyeria química [Àrees temàtiques de la UPC] Albumins Molecule Denaturation (biochemistry) Dinàmica molecular Bovine serum albumin Ion transporter biology Intermolecular force Proteins General Chemistry 021001 nanoscience & nanotechnology 0104 chemical sciences lcsh:QD1-999 chemistry Porin biology.protein Biophysics Lysozyme 0210 nano-technology Proteïnes |
| Zdroj: | ACS Omega Recercat. Dipósit de la Recerca de Catalunya instname UPCommons. Portal del coneixement obert de la UPC Universitat Politècnica de Catalunya (UPC) ACS Omega, Vol 3, Iss 7, Pp 7856-7867 (2018) Lopes Rodrigues, M, Puiggalí-Jou, A, Marti-Balleste, D, del Valle, L J, Michaux, C, Perpète, E & Alemán, C 2018, ' Thermomechanical Response of a Representative Porin for Biomimetics ', ACS Omega, vol. 3, no. 7, pp. 7856-7867 . https://doi.org/10.1021/acsomega.8b00463 |
| ISSN: | 2470-1343 |
| Popis: | The thermomechanical response of Omp2a, a representative porin used for the fabrication of smart biomimetic nanomembranes, has been characterized using microcantilever technology and compared with standard proteins. For this purpose, thermally induced transitions involving the conversion of stable trimers to bigger aggregates, local reorganizations based on the strengthening or weakening of intermolecular interactions, and protein denaturation have been detected by the microcantilever resonance frequency and deflection as a function of the temperature. Measurements have been carried out on arrays of 8-microcantilevers functionalized with proteins (Omp2a, lysozyme and bovine serum albumin). To interpret the measured nanofeatures, the response of proteins to temperature has been also examined using other characterization techniques, including real time wide angle X-ray diffraction. Results not only demonstrate the complex behavior of porins, which exhibit multiple local thermal transitions before undergoing denaturation at temperatures higher than 105 °C, but also suggest a posttreatment to control the orientation of immobilized Omp2a molecules in functionalized biomimetic nanomembranes and, thus, increase their efficacy in ion transport. |
| Databáze: | OpenAIRE |
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