Enrichment of midsized RNAs with manganese chloride precipitation
| Autor: | Paul Freidhoff, Michael F. Bruist, Dillon Friday, Tilman Baumstark |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Viroid Total rna Biophysics chemistry.chemical_element Manganese Biochemistry Primer extension 03 medical and health sciences Chlorides RNA analysis Chemical Precipitation Molecular Biology DNA Primers biology Precipitation (chemistry) Fungi RNA RNA Fungal Cell Biology biology.organism_classification Yeast Viroids 030104 developmental biology chemistry Manganese Compounds RNA Viral Transcription Initiation Site |
| Zdroj: | Analytical biochemistry. 560 |
| ISSN: | 1096-0309 |
| Popis: | Enrichment of specific RNAs is important for RNA analysis. MnCl2 has been used previously to enrich viroid RNA fractions from total RNA from infected plants. We have expanded this method to show that MnCl2 can enrich single-stranded as well as structured RNAs of 450 nt and below from a total RNA preparation. We have applied this method to map the transcription start sites of a PSTVd transcript from total RNA from yeast under conditions where the RNA was previously undetectable. |
| Databáze: | OpenAIRE |
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