| Autor: |
Olivier De Wever, Hannelore Denys, Veronique Cocquyt, Marc Bracke, Christian P. Gespach, Philippe Valet, Catherine Muller, Camille Lehuédé, Dawn Maynard, Christian Vanhove, Jo Vandesompele, Pieter Mestdagh, Ridha Limame, Rudy Van Den Broecke, Geert Braems, Mieke Van Bockstal, Kathleen Lambein, An Hendrix, Lore Lapeire |
| Rok vydání: |
2023 |
| DOI: |
10.1158/0008-5472.22403484.v1 |
| Popis: |
Upper panels: Western blot analysis of E-cadherin, beta-catenin, vimentin, fibronectin, pancytokeratin and estrogen receptor alpha (ERα). Left panel: MCF-7 cells treated for 48h with control medium or CMCAAT. Right panel: MCF-7 cells stably transfected with GFP or OSM cDNA, or treated for 48h with rhOSM (1ng/ml). Tubulin serves as an internal control. Lower left panel: Confocal images of the F-actin cytoskeleton (red), nuclear staining DAPI (blue) and merged signals in MCF-7 cells treated for 48h with control medium or CMCAAT (scale bar is 20 μm) and confocal images of E-cadherin (green), beta-catenin (red) and merge signals with DAPI in MCF-7 cells treated for 48h with control medium or CMCAAT (scale bar is 10 μm). Lower right panel: Immunohistochemical staining of MCF-7-GFP and MCF-7-OSM tumor xenografts for E-cadherin, vimentin, pancytokeratin and ERα (scale bar is 25 µm). |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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