Autoimmune Regulator Is Expressed in the Cells Regulating Immune Tolerance in Thymus Medulla

Autor: Vladimir Ovod, A Ranki, Stylianos E. Antonarakis, Immo Rantala, Juha Tuukkanen, Markku Nieminen, Kentaro Nagamine, Hamish S. Scott, Anssi Lagerstedt, Pärt Peterson, Kai Krohn, Maarit Heino, Nobuyoshi Shimizu, Jun Kudoh
Rok vydání: 1999
Předmět:
Cytoplasm
Cytoskeleton/drug effects/metabolism
Fluorescent Antibody Technique
Microtubules
Biochemistry
Colchicine/pharmacology
Immune tolerance
Cytoskeleton
In Situ Hybridization
Liver/chemistry/cytology/embryology/metabolism
ddc:616
U937 Cells
Autoimmune polyendocrinopathy
Transcription Factors/genetics/ metabolism
Autoimmune regulator
Immunohistochemistry
Spleen/chemistry/cytology/metabolism
Dendritic Cells/chemistry/metabolism
Cell biology
medicine.anatomical_structure
Liver
Cytoplasm/chemistry
Cytochalasin B
Biophysics
Lymph Nodes/chemistry/cytology/metabolism
Spleen
Thymus Gland
In situ hybridization
Cytochalasin B/pharmacology
Biology
Transfection
Clonal deletion
Immune Tolerance
medicine
Humans
Epithelial Cells/chemistry/metabolism
Molecular Biology
Medulla
Cell Nucleus
Microtubules/drug effects/metabolism
Epithelial Cells
Dendritic Cells
Cell Biology
medicine.disease
Autoimmune polyendocrine syndrome type 1
Hela Cells
Thymus Gland/chemistry/ cytology/immunology/metabolism
Immunology
Lymph Nodes
Biological Markers/analysis
Cell Nucleus/chemistry
Colchicine
Biomarkers
HeLa Cells
Transcription Factors
Zdroj: Biochemical and Biophysical Research Communications, Vol. 257, No 3 (1999) pp. 821-825
ISSN: 0006-291X
DOI: 10.1006/bbrc.1999.0308
Popis: The AIRE gene (autoimmune regulator), coding for a putative transcriptional regulatory factor, is mutated in autoimmune-polyendocrinopathy-candidiasis ectodermal dystrophy (APECED). We have investigated the expression of the AIRE gene by mRNA in situ hybridization and immunohistochemistry in various human tissues. Here we show that AIRE is expressed in distinct cells in thymus medulla, and also in rare cells in lymph node paracortex and medulla, and in spleen and fetal liver, but not in the target organs of autoimmune destruction. Double immunofluorescence studies revealed that in thymus medulla both epithelial (cytokeratin positive) and non-epithelial cells expressed AIRE. Subcellularly, AIRE was localised in nuclear dots in thymus and lymph node and also in transfected cells. The cellular localisation of AIRE and its nuclear localisation, compatible with its predicted protein domains, suggest that AIRE may regulate the mechanisms involved in the induction and maintenance of immune tolerance.
Databáze: OpenAIRE
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