Identification of a peptide binding to cancer antigen Kita‐kyushu lung cancer antigen 1 from a phage‐display library
| Autor: | Lin Li, Xiaotong Chen, Jia Wei, Fangcen Liu, Jiayao Yan, Baorui Liu, Xiaoxiao Yu, Lixia Yu |
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| Rok vydání: | 2021 |
| Předmět: |
molecular probe
Cancer Research Biodistribution Phage display Peptide Peptide binding Peptides Cyclic Epitopes Mice Antigen Antigens Neoplasm Peptide Library Stomach Neoplasms In vivo Cell Line Tumor medicine Animals Humans Bacteriophages Tissue Distribution Molecular Targeted Therapy chemistry.chemical_classification gastric cancer KK‐LC‐1 Cancer Original Articles Sequence Analysis DNA General Medicine targeted therapy medicine.disease Drug Discovery and Delivery Oncology chemistry targeting peptide Organ Specificity Cancer research Original Article Ex vivo |
| Zdroj: | Cancer Science |
| ISSN: | 1349-7006 1347-9032 |
| DOI: | 10.1111/cas.15109 |
| Popis: | Kita‐kyushu lung cancer antigen 1 (KK‐LC‐1) is a kind of cancer‐testis antigen with anti‐tumor potential for clinical application. As a class of small‐molecule antigen conjugate, tumor‐targeting peptides have broad application prospects in gastric cancer diagnosis, imaging, and biological treatment. Here, we screened specific cyclic nonapeptides from a phage‐display library. The targeting peptide with the best affinity was selected and further verified in ex vivo tissue sections. Finally, enrichment of targeting peptides in tumor tissues was observed in vivo, and the dynamic biodistribution process was also observed with micro‐positron emission tomography (micro‐PET)/computed tomography (CT) imaging. Studies showed that the specific cyclic nonapeptide had a high binding capacity for KK‐LC‐1 protein. It has a strong affinity and specificity for KK‐LC‐1‐expressing positive tumor cells. Targeting peptides were significantly enriched at tumor sites in vivo, with very low normal tissue background. These findings demonstrated that the KK‐LC‐1 targeting peptide has high clinical potential. The first publicly reported Kita‐kyushu lung cancer antigen 1 targeting peptide. The peptides showed excellent specificity in PET/CT. |
| Databáze: | OpenAIRE |
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