ICMSF Methods Studies. XVI. Comparison of Salt Polymyxin Broth with Glucose Salt Teepol Broth for Enumerating Vibrio parahaemolyticus in Naturally Contaminated Samples
| Autor: | H. Nakanishi, G. Jarvis, Rita R. Colwell, P. West, R. Sakazaki, K. Takizawa, K. Tamura, S. Sakai, L. Beuchat, R. Twedt, Y. Asakawa, G. Barrow, T. M. Gleeson, H. Pivnick, S. Stavric, C. Vanderzant, R. Gray, M. Goddard |
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| Rok vydání: | 1986 |
| Předmět: |
Thiosulfate
chemistry.chemical_classification Sucrose medicine.drug_class Polymyxin Sodium Vibrio parahaemolyticus food and beverages chemistry.chemical_element Salt (chemistry) Biology biology.organism_classification Microbiology Vibrio chemistry.chemical_compound chemistry Most probable number medicine Food Science |
| Zdroj: | Journal of Food Protection. 49:773-780 |
| ISSN: | 0362-028X |
| DOI: | 10.4315/0362-028x-49.10.773 |
| Popis: | Two media, glucose salt teepol broth (GSTB) and salt polymyxin broth (SPB), were compared for their efficacy in enumerating Vibrio parahaemolyticus in naturally contaminated samples using the most probable number (MPN) procedure. Eleven laboratories in four countries participated, six of them using two analysts. One hundred ninety-six of 335 samples were found to contain V. parahaemolyticus . Neither enrichment medium was superior, and both media appeared necessary to prevent false-negative results. There was a high (52.5%) percentage of MPN patterns that were improbable with respect to Poisson distribution in those MPN tubes that yielded V. parahaemolyticus . Suspect colonies that developed on thiosulfate citrate bile salts sucrose (TCBS) plates streaked from GSTB were confirmed (1,438/1,929 = 75%) about as frequently as when streaked from SPB (2,155/3,038 = 71%). |
| Databáze: | OpenAIRE |
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