Increases in airway eosinophilia and a th1 cytokine during the chronic asymptomatic phase of asthma
| Autor: | Zak Callaway, Jungi Choi, Chang Keun Kim, Hirohito Kita, Koji Iijima, Gerald W. Volcheck |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Adult
Chemokine CCL11 Male Eotaxin Pulmonary and Respiratory Medicine medicine.medical_treatment Eosinophil Asymptomatic Article Interferon-gamma Young Adult immune system diseases Forced Expiratory Volume Eosinophilia medicine Humans Chemokine CCL5 Cytokine CCL11 Aged Asthma business.industry Interleukins Sputum Granulocyte-Macrophage Colony-Stimulating Factor Middle Aged respiratory system medicine.disease Immunohistochemistry respiratory tract diseases Eosinophils Intrinsic asthma medicine.anatomical_structure Case-Control Studies Immunology Cytokines Female medicine.symptom business |
| Zdroj: | Respiratory Medicine. 104(10):1436-1443 |
| ISSN: | 0954-6111 |
| DOI: | 10.1016/j.rmed.2010.03.023 |
| Popis: | Summary Background Studies using allergen challenge models have suggested Th2 cytokines promote airway inflammation in asthma. We assessed mediators of airway inflammation during the chronic asymptomatic phase of asthma. Methods Nine non-atopic asthma (NAA) patients, 19 atopic asthma (AA) patients, 20 atopic controls (AC), and 38 normal controls (NC) underwent sputum induction while asymptomatic. Sputum total cell counts and differentials were determined; levels of cytokines IL-4, IL-5, IL-13, GM-CSF, and IFN-γ, and chemokines eotaxin (CCL11) and RANTES (CCL5) were measured by ELISA; and levels of eosinophil-derived neurotoxin (EDN) were measured by radioimmunoassay. Results NAA patients showed higher % eosinophils and total eosinophils compared to AA. NAA and AA patients showed higher IFN-γ and EDN levels compared to AC and NC, with no differences in IL-4, IL-5, or IL-13 levels among the four groups. GM-CSF levels were higher in AA patients compared to AC or NC. In NAA, AA, and AC patients, % eosinophils and EDN levels correlated positively with IFN-γ, GM-CSF, eotaxin, and RANTES, but not with IL-5 levels. Conclusions Baseline airway inflammation of intrinsic and extrinsic asthma is characterized by eosinophilic inflammation and the Th1 cytokine, IFN-γ. GM-CSF, instead of IL-5, and chemokines may coordinate airway eosinophilia during the chronic asymptomatic phase of asthma. |
| Databáze: | OpenAIRE |
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